BED捕获酶联免疫法检测广西某吸毒监测哨点HIV新近感染率

Detection of HIV incidence using BED capture enzyme immunoassay at a surveillance sentinel site of injection drug users in Guangxi

目的了解广西某吸毒监测哨点注射吸毒人群（injection drugusers，IDU）的HIV新近感染情况，估算HIV新近感染率。方法对广西某吸毒监测哨点2005—2007年787份血清样本进行监测，以酶联免疫吸附试验（ELISA）筛检出168份HIV-1阳性样本，再经BED捕获酶联免疫法（BED capture enzyme immunoassay，BED．CEIA）检测出其中新近感染的样本共17份，获得该人群HIV-1的新近感染率。结果787份监测样本中共筛检出HIV-1确认阳性样本168份，新近感染样本17份。该哨点2005—2007年HIV感染率分别为23．06％（92／399）、21．90％（46／210）、16．85％（30／178），新近感染率分别为7．41％、6．94％、3．12％。结论在广西某吸毒监测哨点IDU中，2005—2007年HIV新近感染率绝对数值有所降低，已发现的HIV感染者可能部分为既往感染者。

Objective To detect recent HIV incidence of injection drug users （IDU） from a surveillance site of IDU in Guangxi and estimate HIV incidence. Methods 787 Samples from a IDU surveillance sentinel site in Guangxi （2005-2007） were collected for ELISA and 168 were HIV-1 positive; then 168 HIV-1 positive samples were detected by BED capture enzyme immunoassay （BED-CEIA） and 17 were positive. Results A total of 787 samples were tested and 168 were HIV-1 positive and 17 were BED- CEIA positive. The prevalence rates of the IDU surveillance sentinel site were 23.06% （92/399） ,21.90% （46/210） and 16. 85% （ 30/178 ）, and the incidence rates were 7.41% （ 10/135 ）, 6. 94% （5/72） and 3. 12% （2/64） respectively from 2005 to 2007. Conclusion The incidence rate of HIV infections of IDU from a surveillance site of IDU in Guangxi appeared to decline slowly in recent years, and some might have been infected for long time.