PTEN、P16和nm23-H1在非小细胞肺癌中的表达及临床意义

The expression and clinical significance of PTEN,P16 and nm23-H1 in non-small cell lung cancer

目的研究非小细胞肺癌(non-small cell lung cancer,NSCLC)中抑癌基因PTEN、p16和转移抑制基因nm23-H1的表达及意义。方法应用免疫组织化学S-P法检测84例NSCLC手术切除标本及25例癌旁组织中PTEN、p16和nm23-H1蛋白的表达,并分析其与临床病理因素的关系。结果PTEN、p16和nm23-H1蛋白在NSCLC中的阳性表达率均较癌旁组织降低(48.8%vs 92.0%、41.7%vs 88.0%、56.0%vs 84.0%);I期肺癌患者三种蛋白的表达均显著高于II、III期;PTEN和p16的表达与NSCLC的肿瘤分化和淋巴结转移相关,但nm23-H1与肿瘤分化和淋巴结转移无关;三种蛋白同时失表达与患者3年生存率显著相。结论PTEN、p16和nm23-H1蛋白同时表达缺失在NSCLC发生发展过程中起着重要作用,联合检测可做为预测肺癌患者预后的有效指标。

Objective： To investigate the expression and clinical significance of tumor suppressor gene PTEN,p16 and metastasis suppressor gene nm23-H1 protein in tumourigenesis and development in NSCLCs.Methods： Immunohistochemical SP staining was performed on 84 NSCLCs and 25 corresponding adjacent tissues to detect the expressions of PTEN,p16 and nm23H-1 proteins.The results of the expression pattern were analyzed with clinicopathological parameters and patient survival.Results： The positive rate of PTEN,p16 and nm23-H1（48.8% vs 92.0%、41.7% vs 88.0%、56.0% vs 84.0% respectively） were significantly lower than that in NSCLCs compared with adjacent lung tissues.The immunohistochemical expression of these proteins was significantly higher in stage I compared with stage II and III.The expression of PTEN and p16 was correlated with tumor differentiation and positive lymph node status,but no correlation was observed for nm23-H1.All the loss of expression of the three proteins was linked to distant metastasis and predicted shortened survival in NSCLCs.Conclusion： Co-downregulation of PTEN,p16 and nm23-H1 at protein level plays an important role in the evolution and progression of NSCLCs.And the co-expression of these three proteins may be an independently prognostic predictor of NSCLC and has important clinical significance.