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大鼠脑缺血再灌注后组织蛋白酶D蛋白质及mRNA的表达 被引量：1

Expression of the protein and mRNA of CathepsinD in Rats after Cerebral Ischemia-Reperfusion
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摘要 目的:探讨大鼠缺血再灌注后溶酶体组织蛋白酶D(Cathepsin DCD)在不同时段蛋白质及mRNA表达变化。方法:将60只SD大鼠随机分为三组:正常组(10只),假手术组(10只),脑缺血再灌注组(模型组)(40只),线栓法制备大脑中动脉梗死模型(MCAO),免疫组化及RT-PCR法分别检测CathepsinD的蛋白和mRNA表达。结果:与正常组和假手术组比较,模型组在大鼠脑缺血再灌注损伤后6hCathepsin D的蛋白和mRNA表达明显增强(P<0.05),24h达高峰,48h仍保存高水平。结论:Cathepsin D在大鼠脑缺血再灌注后表达增强,溶酶体CathepsinD可能参与了脑缺血再灌注损伤后神经细胞凋亡。 Objective： To study the changes of the protein and mRNA of Cathepsin D expression in different time point in rat after cerebral ischemia-reperfusion. Methods： 60 SD rats were randomly divided into three groups： normal group （n=10）,sham-operation group （n=10）,cerebral ischemia-reperfusion group （n--40）, prepared by suture middle cerebral artery infarction model （MCAO）,the expression of the protein and mRNA of Cathepsin D were respectively evaluated by methods of immunohistochemistry and RT-PCR. Results： Compared with normal group and sham-operated group, The expression level of the protein and mRNA of Cathepsin D in the rats of model group increased significantly 6 hour and got to their peak 24 hour, kept a high level on 48h. Conclusions： The expression of Cathepsin D after ischemia-reperfusion is high, Cathepsin D may be involved in the process of neuronal apoptosis in the brain after ischemia-reperfusion injury.
作者 刘柳青 张智博 龚晓燕
机构地区 南华大学 长沙市第一医院神经内科
出处 《现代生物医学进展》 CAS 2009年第20期3868-3870,共3页 Progress in Modern Biomedicine
关键词 脑缺血再灌注 溶酶体 CATHEPSIN D cerebral ischemia-reperfusion lysosome Cathepsin D
分类号 R692 [医药卫生—泌尿科学]
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参考文献15

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同被引文献23

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现代生物医学进展
2009年 第20期

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