蛹虫草培养液成分研究Ⅱ

Chemical Constituents of Culture Liquid of Cordyceps Mlitaris (L.) Link.

目的:研究蛹虫草人工培养液的化学成分。方法:利用硅胶柱层析、制备薄层、SephadexLH-20、重结晶等方法分离、纯化单体化合物,并根据理化性质及光谱数据鉴定其化学结构。结果:分离并鉴定了8个化合物,分别为3,7,8-三甲基-异咯嗪(1);琥珀酸(2);胸腺嘧啶(3);虫草素(4);腺苷(5);2吲哚乙丁二酰胺(6);1(-5-羟甲基)-呋喃-3-羧基-β-咔啉(7);5(-1-甲基丙基)-3,6-氧代-2-哌嗪乙酰胺(8)。结论:化合物1,6,7为首次从虫草属真菌中分离得到。

Objective： To investigate the chemical constituents of the culture liquid of Cordyceps militaris （Fr.） Link. Methods： The chemical constituents of C. militaris were isolated and purified by the chromatographic methods such as column chromatography of silica gel, SephadexLH-20, and recrystallization, and then their structures were identified by physicochemical properties and spectral analysis. Results： ight known compounds were identified as, 7,8-trimethyl-iso-alloxazin （1）, succinic acid （2）, thymine （3）, cordycepin （4）, adenosine （5）, succinamide,N,N＇-bis-[2-（3-indol）-ethyl] （6）, Flazin （7） and 5-（1- Methylpropyl ）-3,6-dioxo-2-piperazine acetamide （8）. respectively, on the basis of NMR spectra. Conclusion： compounds 1, 6 and 7 were isolated from Cordyceps for the first time.