放射性肺损伤小鼠动物模型的建立与鉴定

Establishment and identification of mouse model of radiation-induced lung injury

目的:建立放射性肺损伤小鼠动物模型和有效的定量评价方法。方法:雄性昆明种小鼠36只,随机分作2组,对照组(12只)不做任何处理,照射组(24只)全肺单次照射18Gy。于照射后2、6和12周,进行小鼠肺部螺旋CT扫描及图像分析,获得小鼠全肺的平均灰度值;采用HE染色法观察肺组织的变化。结果:照射后2、6和12周的CT图像分析表明,与正常对照组相比,照射组CT平均灰度值均上升,照射后2和6周差异有统计学意义,P值分别为0.003、0.006。与对照组相比,照射组小鼠的肺组织在照射后存在明显的病理组织学上的改变,早期以急性炎症为主,表现为肺充血、水肿、肺间质增厚,后期损伤以肺泡间隔的进行性纤维化为特征。结论:小鼠全肺单次照射18GyX射线可建立放射性肺损伤小鼠动物模型,用螺旋CT扫描,计算机软件UTHSCSA Image Tool3.0灰度分析方法可以有效地定量评价早期放射性肺损伤程度。

OBJECTIVE：To establish a mouse model of radiation-induced lung injury and the effective assessment to evaluate the model. METHODS：Thirty six Kunming mice were randomly classified into two groups,one received sham-irradiation （control group,Group C,n=12） and another received irradiation on the thoraces with single fraction 18-gray X-rays （treatment group,Group X,n=24）. Spiral computed tomograph scanning and image analysis of the mean gray value were carried out to acquire the data of each mouse after the irradiation for 2,6 and 12 weeks. The pathologic changes of lung were studied by the way of HE staining pathologic slices of the lung. RESULTS：The analysis of mean gray value：2 ,6 and 12 weeks after the irradiation,compared with Group C,the mean gray values of Group X all increased,and for 2 weeks （P=0.003） and 6 weeks （P=0.006）,the value increased obviously. The analysis of HE staining pathologic slices of the lung：compared with Group C,significant histological and pathologic changes were observed. CONCLUSION：It is possible to establish the mouse model of radiation-induced lung injury with 18-gray X-rays of the whole thorax,and the new way of image analysis,CT and UTHSCSA Image Tool 3.0 grayscale analysis,can efficiently evaluate the degree of the lung injury.