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The Arabidopsis PARAQUAT RESISTANT2 gene encodes an S-nitrosoglutathione reductase that is a key regulator of cell death 被引量:19

The Arabidopsis PARAQUAT RESISTANT2 gene encodes an S-nitrosoglutathione reductase that is a key regulator of cell death
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摘要 Metabolism of S-nitrosoglutathione (GSNO), a major biologically active nitric oxide (NO) species, is catalyzed by the evolutionally conserved GSNO reductase (GSNOR). Previous studies showed that the Arabidopsis GSNOR1/ HOT5 gene regulates salicylic acid signaling and thermotolerance by modulating the intracellular S-nitrosothiol level. Here, we report the characterization of the Arabidopsisparaquat resistant2-1 (par2-1) mutant that shows an anti-cell death phenotype. The production of superoxide in par2-1 is comparable to that of wild-type plants when treated by paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride), suggesting that PAR2 acts downstream of superoxide to regulate cell death. PAR2, identified by positional cloning, is shown to be identical to GSNOR1/HOT5. The par2-1 mutant carries a missense mutation in a highly conserved glycine, which renders the mutant protein unstable. Compared to wild type, par2-1 mutant has a higher NO level, as revealed by staining with 4,5-diaminofluorescein diacetate. Consistent with this result, wild-type plants treated with an NO donor display resistance to paraquat. Interestingly, the GSNOR1/HOT5/PAR2 protein level, other than its steady-state mRNA level, is induced by paraquat, but is reduced by NO donors. Taken together, these results suggest that GSNOR1/HOT5/PAR2 plays an important role in regulating cell death in plant cells through modulating intracellular NO level.
出处 《Cell Research》 SCIE CAS CSCD 2009年第12期1377-1387,共11页 细胞研究(英文版)
基金 We thank Dr Gary Loake (University of Edinburgh, UK) for providing gsnor1-3 seeds. We are grateful to Drs Chuanyou Li, Shuhua Yang and Yiqin Wang for critically reading the manuscript. This study was supported by grants from the National Natural Science Foundation of China (30330360), the Ministry of Science and Technology of China (2006AA 10A 112) and the Chinese Academy of Sciences (KSCX2-YW-N-015).
关键词 GSNOR1/HOT5/PAR2 nitric oxide PARAQUAT cell death superoxide 谷胱甘肽还原酶 细胞死亡 百草枯 拟南芥 亚硝基 基因编码 蛋白质水平 mRNA水平
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