硬膜外腔植入髓核对大鼠脊髓背根神经节SP和CGRP表达的影响

Effect of nucleus pulposus allografted into epidural space on SP and CGRP expressions of DRG neurons in rats

目的观察硬膜外腔植入异体髓核对大鼠L6-S1脊髓背根神经节细胞SP和CGRP表达的影响,以期为椎间盘源性疼痛发病机制提供细胞生物学基础。方法18只雄性SD大鼠(体重260～280g)随机分为三组:脂肪组、髓核组、假手术组,每组n=6。另外3只雄性SD大鼠用来提供异体脂肪和髓核。术后第30d取L4-L6脊髓背根神经节和脊髓背角,采用免疫组织化学染色方法观察髓核对脊髓背角和背根神经节细胞肽类神经递质SP和CGRP表达的影响。结果术后30d脂肪组大鼠与假手术组相比,脊髓背角和背根神经节细胞SP和CGRP表达没有显著性差异(P>0.05);髓核组与脂肪组和假手术组相比脊髓背角和背根神经节细胞SP和CGRP表达显著增加(P<0.05)。结论硬膜外腔植入异体髓核可引起脊髓背根神经节细胞SP和CGRP表达增加。

Objective To obseve the effects of SP and CGRP expression of dorsal root ganlion neurons in rats of nucleus pulposus allografted into epidural space, so as to provide theoretical ev- idence for the treatment of collagenase on discogenic pain. Methods Eighteen male Sprague-Dawley rats were randomly divided into three groups with 6 rats in each： sham-operated group,fat group and nucleus pulposus group. The allografted fat and nucleus pulposus were provided by the other 3 male SD rats. Rats in the sham-operated group were given an epidural space exposure without any graft implantation. In the fat group and nucleus pulposus group,rats had allografls of adipose tissue（approximately 4.0mg） and nucleus pulposus（approximately 3.0-4.0mg） implanted into epidural space,respectively. 30 clays postoperatively,the expressions of SP and CGRP in L4-5 Dorsal Root Ganglion （DRG） neurons were detected by immunohistochemistry method. In addition,the structure of epidural space were observed. Results 30 clays postoperatively,the SP and CGRP expressions in the sham-operated group and fat group were not significantly different （P〉0.05）. The SP and CGRP expressions of DRG neurons in nucleus pulposus group were significantly increaeed compared with sham-operated group,fat group （P〈0.05） respectively. Conclusion The SP and CGRP expressions of DRG neurons in rats can increase because nucleus pulposus were allografied into the epidural space.