ApoE基因多态性、hs-CRP与冠心病的相关性研究

The correlation of the polymorphism of apolipoprotein E and high sensitivity C-reactive protein for coronary heart disease

目的分析载脂蛋白E(ApoE)的基因多态性、高敏C-反应蛋白(hs-CRP)含量与冠心病的相关性。方法采用酚氯仿抽提核酸法从凝血块中分离,应用聚合酶链反应(PCR),Hha1内切酶消化法对94例冠心病患者和100例健康对照组进行ApoE基因多态性进行检测;采用生化全自动仪,以乳胶增强免疫透射比浊法检测血清hs-CRP含量。结果①ApoE等位基因频率:冠心病组与对照组比较,ε2等位基因频率明显降低(P<0.01),ε4等位基因频率升高(P<0.005);②血清hs-CRP浓度,冠心病组显著升高(P<0.05),但不同等位基因之间,hs-CRP无显著性差别(P>0.05)。结论联合监测ApoE基因多态性及血清hs-CRP含量,对心血管疾病危险性的预测是敏感及有价值的指标。

Objective To analyze the polymorphism of apolipoprotein E（ApoE）,and high sensitivity C-reactive protein（hs-CRP） levels and the relevance of coronary heart disease（CHD）.Methods Phenol chloroform extraction was used to get the DNA from the isolated blocks blood coagulation.Polymerase chain reaction,Hhal endonuclease digestion were used to test the 94 cases of CHD patients and 100 healthy control group ApoE polymorphism.Hs-CRP level was measured by immune latex transmission turbidity.Results ① ApoE allele frequency：compared with the control group,ε2 allele frequency decreased significantly（P=0.01） in CHD group,ε4 allele frequency increased（P=0.005）.② Serum hs-CRP concentration in CHD group was significantly higher than that of control grooup（P〈0.05）,but there was no significant difference between different alleles on hs-CRP.Conclusions The ApoE gene polymorphism and serum hs-CRP are the sensitive and valuable indicators,which can predict the the risk of cardiovascular disease.