SPK/S1P信号途径对人肝癌细胞增殖、凋亡的作用

Effects of the SPK/S1P signal on proliferation and apoptosis of human hepatoma cell HepG2

目的探讨鞘氨醇激酶/1-磷酸鞘氨醇信号途径(SPK/S1P)对人肝癌细胞增殖、凋亡的影响。方法体外培养人肝癌细胞株HepG2,观察5～20μmoL/L浓度范围鞘氨醇激酶的特异性抑制剂N,N′-二甲基鞘氨鞍醇(DMS)对HepG2细胞增殖、凋亡的影响。结果MTT实验结果显示,5～20μmoL/L浓度范围的DMS对细胞增殖均有抑制作用,透射电镜观察对照组细胞可见细胞凋亡的变化,TUNEL标记法检测处理组阳性细胞表达百分数较对照组明显增多(P<0.01)。结论DMS对HepG2细胞的生长增殖具有抑制作用,并促其发生凋亡。

Objective To investigate the effect of the SPK/S1P signal on proliferation and apoptosis in human hepatoma cells.Methods Treated with 5-20 μmol/L dimethyl sphingosine（DMS） in culture medium,human hepatoma cell HepG2 was determined using MTT assay,a scanning electron microscope and TUNEL staining.Results MTT assay demonstrated that DMS inhibited the growth of human hepatocarcinoma HepG2 cells in a time-and dose-dependent manner.HepG2 cells showed typical morphological characters of apoptosis.TUNEL demonstrated that the stained cells of the DMS group were much more than those of the control group,which had significant differences（P〈0.01）.Conclusion DMS inhibits the growth of human hepatocarcinoma HepG2 cells,and can induce apoptosis of human hepatocarcinoma HepG2 cells.