吉西他滨白蛋白纳米粒对胰腺癌细胞株体外抑制效应的实验研究

Effect of 406 nm-gemcitabine-loaded albumin nanoparticles on in vitro human pancreatic cancer cells CFPAC-1

目的:通过体外实验,探讨406nm吉西他滨白蛋白纳米颗粒(406nm-GEM-NP)对人胰腺癌细胞株CFPAC-1体外增殖的抑制效应。方法:以人胰腺癌细胞株CFPAC-1为研究对象,分为4个给药组:406nm-GEM-NP组、单纯GEM组、空白纳米颗粒组和无药对照组,采用MTT法(四甲基偶氮唑蓝)观察不同药物浓度对肿瘤细胞生长的抑制作用;并同时观察给药后48h和72h肿瘤细胞的增殖抑制率。结果:MTT试验检测提示406nm-GEM-NP和GEM均具有显著的细胞抑制作用,且406nm-GEM-NP的细胞抑制率在50μg/mLGEM浓度时,超过了单纯GEM(P<0.05),且有明显的时间依赖性,提示有显著的药物缓释作用;空白纳米颗粒对细胞抑制轻微,且不具有时间和浓度依赖性。结论:406nm-GEM-NP对人胰腺癌细胞株CFPAC-1具有显著的体外细胞增殖抑制作用。

Objective To investigate the anti-proliferation effect of 406 nm-gemcitabine （GEM）-loaded albumin nanoparticles on human pancreatic cancer cells, CFPAC-1, in vitro. Methods CFPAC-1 cells were treated with 406 nm-GEM-loaded albumin nanoparticles, pure GEM, blank albumin nanoparticles and culture fluid respectively. CFPAC-1 cell growth inhibition rates in different groups were detected by M3W essay; and also, the growth inhibition rates were detected at 48 h and 72 h after drug administration. Results The 406 nm-GEM-NP group and GEM group produced significant anti-proliferation effect on CFPAC-1 cell gruwth. The 406 nm-GEM-NP group had higher inhibitory effect than the GEM group using the concentration of 50 μg/mL GEM（P〈0.05）, and its inhibitory effect at 72 hours was much higher than that of at 48 hours. Blank albumin nanoparticles produced trifle inhibition on the CFPAC-1 cells without correlation to time or concentration. Conclusions 406 nm-GEM-NP has a significant anti-proliferation effect on the pancreatic cancer cells CFPAC-1 with time-dependence in vitro.