塞来西布对食管癌ECA-109细胞系放射增敏效应的研究

Radiosensitizing effect of the selective cyclooxygenase-2 inhibitor celecoxib on esophageal carcinoma cell line ECA109

目的:了解环氧化物酶-2(COX-2)选择性抑制剂——塞来西布对食管癌ECA-109细胞系的放射增敏作用。方法:选择ECA-109细胞系作为试验对象,检测ECA-109细胞系的COX-2表达水平,设塞来西布0.25、50、100μmol/L4个实验组,分别予6MV-X线照射0、2、4、6Gy后,再分别行MTT比色试验、克隆形成实验、流式细胞仪计数,检测细胞的增殖能力、克隆形成能力及凋亡3项指标。结果:不同辐射剂量与不同浓度的塞来西布对ECA-109细胞的增殖、凋亡率作用有统计学差异(P<0.05),塞来西布与放射对ECA-109细胞的增殖抑制和诱导凋亡作用随塞来西布的浓度和照射剂量的增加而增强;塞来西布联合放射对ECA-109细胞的存活率、D0值和Dq值作用均小于单纯放射组,Dq变小;塞来西布和4Gy(6MV-X)联合用于ECA-109细胞其凋亡率有统计学差异(P<0.05)。结论:塞来西布能抑制ECA-109细胞增殖,诱导其凋亡。塞来西布与放射联合应用对ECA-109细胞的增殖抑制、诱导凋亡有协同增强的作用。

AIM：To investigete the radiosensitizing effect of celecoxib on esophageal carcinoma cell line CEA109.METHODS：The expression of COX-2 in CEA109 cells was detected by immunohistochemical staining.The MTT assay and colony forming assay were performed to determine the influence of celecoxib on growth and proliferation of esophageal carcinoma cell line CEA109.The apoptosis of CEA109 cells was determined by flow cytometry.RESULTS：There were differences in colony forming,cell proliferation,and stage apoptosis among different dose levels of radiation and celecoxib concentrations （all P〈0.05）.And the inhibition of proliferation and induction of apoptosis in CEA109 cell line were depend on the dose levels of radiation and celecoxib concentrations.CEA109 cell survival rate was lower after treatment with the combination of radiation and celecoxib than that of radiation alone.The apoptosis rate of combined use of celecoxib（50 μmol/L） and radiation（4Gy） was significant different from the apoptosis rate of simple radiation group（P〈0.05）.CONCLUSION：Celecoxib can inhibit the proliferation and induce the apoptosis of ECA-109 cell line.Radiation combined with celecoxib has synergistic effect on ECA-109 cell line proliferation and apoptosis.Celecoxib can enhance the sensitivity of ECA-109 cell line to irradiation.