牛POMC基因多态性及其与南阳牛生长性状的相关分析

The polymorphism of bovine POMC gene and its association with the growth traits of Nanyang cattle

为研究阿片黑皮质素前体（POMC）在动物采食和能量平衡调控中发挥重要作用，文章采用PCR—SSCP结合DNA测序方法，对秦川牛、南阳牛、郏县红牛、晋南牛、鲁西牛、安格斯牛和荷斯坦奶牛共计480头个体POMC基因的多态性进行研究，并分析了多态位点与南阳牛生长性状的相关性。结果表明，牛POMC基因3个位点中，在3’侧翼区P3位点新发现3个连锁存在的SNP（811845C〉T、811821T〉C和811797A〉G与NW_928357对照）。POMC基因3’侧翼区多态位点与南阳牛6月龄体重和0-6月龄平均日增重显著相关，BB型个体显著大于AA型（P〈0．05）。

Pro-opiomelanocortin （POMC） plays an important role on animal ingestive behavior and energy homeostasis. Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds （i.e., Qinchuan, Nanyang, Jiaxian Red cattle, Jinnan, Luxi, Angus, and Chinese Holstein）, and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth gaits in Nanyang cattle. Three SNPs （811845 C〉T, 811821 T〉C and 811797 A〉Cx ref. NW_928357） in linkage were detected in P3 locus, which were located at the 3＇ flanking region of bovine POMC gene. The body weight at 6-month-old and the average daily gain of 0-6-month-old were higher in Nanyang cattle with the genotype BB than with the genotype AA （P〈0.05）.