摘要
目的了解+GZ重复暴露后大鼠脑组织c-fos和c-jun基因表达的变化,探讨+GZ引起脑损害的分子机制,为+GZ防护研究提供实验依据。方法24只雄性Wistar大鼠随机分成对照组、+GZ重复暴露后30min、6h和24h四组,每组6只。对照组大鼠G值为+1GZ,实验组大鼠在动物离心机上经历了3次+10GZ/3min(两次间间隔30min)作用。分别于暴露后30min、6h和24h处死大鼠取脑,提取总RNA,用建立的反转录聚合酶链反应(RT-PCR)定量检测方法检测大鼠脑组织c-fos和c-junmRNA表达水平。结果+GZ重复暴露后30min大鼠脑组织c-fos和c-junmRNA明显升高,分别是对照组的2.8倍和1.5倍,但6h和24h时恢复正常。结论+GZ重复暴露可诱发大鼠脑组织c-fos和c-junmRNA的表达,这种表达快速且短暂,在+GZ所致脑损害的病理过程中起一定作用。
Objective To explore the molecular mechanism of brain damage induced by repeated +GZ exposures, and to provide experimental evidence for protecting +GZ induced brain damage. Methods Twentyfour concious Wistar rats served as subjects. They were randomly divided into 4 groups, using an animal centrifuge, control rats (n=6) were exposed to +1 GZ and 3 experimental rats (n=18) were exposed to +10 GZ three times, each for 3 min with 30 min interval in between. The brains were taken 30 min, 6 h and 24 h after the last centrifuge run and total RNA was isolated by singlestep acid guanidinium thiocyanatephenolchloroform extraction method. The mRNA expression levels of cfos and cjun in rat brain were measured by quantitative reverse transcription polymerase chain reaction (RTPCR). Results The cfos and cjun mRNA expression levels in rat brains taken 30 min after repeated +GZ exposures were significantly higher than those of control group, but the differences between the levels of control group and those of experimental rat brain taken 6 h and 24 h after +GZ exposure were nonsignificant. Conclusion It is suggested that cfos and cjun mRNA expression in rat brain can be stimulated by repeated +GZ exposures and the increased expression was brief and transient. The increased expression of cfos and cjun may play a role in the pathologic course of brain damage induced by+GZ exposures.
出处
《中华航空航天医学杂志》
CSCD
1998年第2期77-80,共4页
Chinese Journal of Aerospace Medicine