IFAT和McAb-LAT诊断囊虫病的研究

DIAGNOSIS OF CYSTICERCOSIS BY IFAT AND McAb-LAT

本文采用猪囊尾蚴冰冻切片抗原间接荧光抗体试验（ＩＦＡＴ）和抗猪囊尾蚴单克隆抗体胶乳凝集试验（ＭｃＡｂ－ＬＡＴ）对囊虫病患者进行特异性抗体和抗原的检测，囊虫病患者抗体检出率为８３．７１％，分型抗体检测，混合型及脑型检出率明显高于皮肌型（Ｐ＜０．０１）；患者循环抗原的检出率为７３．０５％，脑型患者囊虫活动期的循环抗原的阳性率明显高于囊虫钙化期（Ｐ＜０．０１），药物治疗３个月以上患者循环抗原的阳性率降为４４．１１％。ＩＦＡＴ和ＭｃＡｂ－ＬＡＴ在囊虫病临床诊断中具有较高价值。

The antibodies of patients with cysticercosis were detcted by indirect fluorecent antibody test(IFAT) using frozen section of cysticercus of Taenia solium. The monoclonal antibodylatex agglutination test (McAbLAT) were applied for the detection os circulating antigens of cysticercosis. Sensitivity in detecting cysticercosis was 83.71% by IFAT and 73.05% by McAbLAT. The antibody positive rate was higher in the cerebral and mixed cysticercosis than that in the subcutaneous and muscular cysticercosis(P<0.01). The detective rate of circulating antigen was higher in the active stage than that in calcific stage (P<0.01). After more than 3 month treatment, the detective rate of circulating antigen was decreased to 44.11%. The results demonstrated that McAbLAT and IFAT were available for the diagnosis of cysticercosis. The detection of circulating antigen was useful for diagnosis of active infection and evaluation of therapeutic effect in cysticercosis. 