压力性尿失禁患者阴道壁组织中MMP-10、TIMP-3和LN的表达及意义

Expression and significance of MMP-10,TIMP-3 and LN in the vaginal wall tissue of the women with stress urinary incontinence

目的观察女性压力性尿失禁(SUI)患者阴道壁组织中基质金属蛋白酶-10(MMP-10)及其抑制剂-3(TIMP-3)和层粘连蛋白(LN)的表达变化,并探讨其意义。方法随机选取40例有完整随访资料的女性SUI患者为SUI组,选取同期30例无压力性尿失禁和盆腔脏器脱垂(POP)的良性妇科病手术患者为对照组,均于术中取患者阴道壁结缔组织,采用免疫组化法检测其中的MMP-10、TIMP-3及LN。结果SUI组MMP-10的表达较对照组明显升高,TIMP-3和LN的表达较对照组明显下降(P<0.05)。SUI组MMP-10与TIMP-3、LN的表达分别呈负相关(r分别为-0.494、-0.459,P均<0.05);TIMP-3与LN的表达呈正相关(r=0.946,P<0.05)。结论SUI中MMP-10升高及TIMP-3和LN的下降,使胶原代谢活跃,对胶原纤维的降解明显增强。联合检测阴道壁组织中的MMP-10、TIMP-3和LN有助于SUI的诊疗。

Objective To investigate the expression and significance of MMP-10,TIMP-3 and LN in the vaginal wall tissue of the women with SUI. Methods Vaginal wall tissues were obtained from 40 patients with SUI were followed-up as study group ,30 women undergoing without SUI and POP as control group. Immunohistochenical technique were applied to detect the expression of MMP-10, TIMP-3 and LN. Results The expression of MMP-10 was significantly higher in SUI group than in control group. The expression of TIMP-3 and LN were significantly lower in SUI group than in control group（ P 〈 0.05）. There are negative relationship between MMP-10 and TIMP-3, MMP-10 and LN respectively. There is positive relationship between TIMP-3 and LN（P 〈 0.05）. Conclusion The increased of MMP-10 and the decreased of TIMP-3 and LN may cause the degradation of collagen in total, and that may play an important role in the development of SUI. Examination of MMP-10,TIMP-3 and LN may have an important significance for the diagnose and therapy of SUI.