回转器模拟失重对SGC-7901、HFE-145细胞增殖和周期的影响

Effects of simulated weightlessness on cell proliferation and cell cycle of cell line SGC-7901 and HFE-145

目的探讨模拟失重对胃癌SGC-7901细胞和胃黏膜上皮HFE-145细胞增殖和周期的影响。方法采用回转器模拟失重,两种细胞各分为两组,回转组和1G对照组,实验时间共72h。采用免疫组化法观察两种细胞增殖核抗原(proliferating cell nuclear antigen,PCNA)表达的变化,应用流式细胞仪测定细胞周期。结果与对照组相比,回转组SGC-7901细胞48h及72hPCNA抗体阳性率明显减少(P<0.05及P<0.01),G0+G1期细胞显著增多(P<0.01及P<0.05);12h、24h及36h PCNA抗体阳性细胞的比率无显著性差异。与对照组相比,回转组HFE-145细胞12hPCNA抗体阳性率明显减少(P<0.05),G0+G1期细胞显著增多(P<0.05)。结论回转器模拟失重使胃癌SGC-7901细胞48h及72h时出现细胞周期阻滞、增殖抑制。胃黏膜上皮HFE-145细胞在12h时出现上述变化。

Objective To investigate the effects of simulated weightlessness on cell proliferation and cell cycle of human gastric carcinoma cell line SGC-7901 and human gastric mucosa cell line HFE-145. Methods A rotating clinostat was used to simulate weightlessness. Every cell line was divided into two groups, one was rotating group, the other was 1G control group. The whole experimental session was 72h. The expressions of PCNA（proliferating cell nuclear antigen） were examined by immunohistochemical stain. The changes of cell cycle were examined by a cytometer. Results Compared with the control group, the cell proliferation of SGC-7901 cell was inhibited in 48h and 72h in the rotating group, and the cell conversion from G1 to S phase was suppressed. Compared with the control group, the cell proliferation of HFE-145 cell was inhibited in 12h in the rotating group, and the cell conversion from G1 to S phase was suppressed. Conclusions the cell proliferation of SGC-7901 cell is inhibited in 48h and 72h during simulated weightlessness by a clinostat, but HFE-145 cells only have present those above-mentioned changes in 12h during simulated weightlessness by a clinostat.