摘要
利用单引物PCR方法获得了甘蓝型油菜APETALA3重复基因拷贝BnAP3-3和BnAP3-4的5′调控序列。分析结果表明,两段调控区序列一致性为80.43%,大多数的保守功能元件如TATA盒和CAAT盒没有明显差异,都含有一些应对环境胁迫及诱导的元件,如MYB1AT、GATABOX和DOFCOREZM等。少数顺式作用元件存在较大差异,其中CGACGOSAMY3、CArG box、CARGCW8GAT及CAREOSREP1为BnAP3-3的5′调控序列所特有,而BnAP3-4的5′调控序列则含有GT1CONSENSUS。半定量RT-PCR显示,BnAP3-3表达量远高于BnAP3-4,可能与二者调控区某些功能元件序列差异有关。
The 5′ regulatory region of two APETALA3 duplicated genes BnAP3-3 and BnAP3-4 in Brassica napus were cloned using single primer PCR.Sequence analysis showed that the two 5′ regulatory region sequences have 80.43% homologous with each other.Most of the conserved cis-regulatory elements were similar,such as TATA-box,CAAT-box and some stress responsive elements including MYB1AT,GATABOX and DOFCOREZM motifs.There were also a few different motifs between them.CGACGOSAMY3,CArG box,CARGCW8GAT and CAREOSREP1 motifs were found in the 5′ regulatory region of BnAP3-3,while the 5′ regulatory region of BnAP3-4 contained GT1CONSENSUS motif.The semi-quantitative RT-PCR demonstrated that the relative expression of BnAP3-3 was significantly higher than that of BnAP3-4.The results indicated that the cause of the expression difference of the two genes may be from the sequence difference of their 5′ regulatory region.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第11期2168-2173,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金资助项目(30571174
30871540)