人β-防御素-2在喉癌组织中的表达及与CD1a＋树突状细胞浸润的相关性

Expression of human beta-defensin-2 in laryngeal squamous cell carcinoma and its correlation with CD1a＋ dendritic cells infiltrating in tumor

目的研究喉癌组织中人β-防御素-2（humanbeta—defensin-2，HBD-2）mRNA和蛋白的表达，探讨其在机体抗肿瘤免疫中的潜在价值。方法采用链霉亲合素-生物素酶复合物法（streptactividin．biotincomplex，SABC）检测34例喉鳞癌石蜡标本中HBD-2蛋白表达及CD1a＋树突状细胞（dendriticcell，DC）的浸润；收集18例喉鳞癌患者术中切除的新鲜肿瘤组织及其中5例距肿瘤边缘2cm的癌旁上皮组织，应用互补DNA（cDNA）探针原位杂交检测HBD-2mRNA的表达。分析HBD-2在不同的临床和病理状态下的表达及与CD1a＋树突状细胞浸润的关系。结果①免疫组化结果显示，在不同的病理分化组中HBD-2的表达差异有统计学意义（F=6．809，P〈0．05），高分化组HBD-2表达水平明显高于中分化和低分化组（P值均〈0．05）。不同临床分型、T分期及有无淋巴转移组中HBD-2的表达水平差异无统计学意义。②原位杂交结果显示，18例喉鳞癌和5例癌旁上皮组织中HBD-2mRNA的表达存在差异，喉癌低分化组明显弱于高分化组和癌旁上皮组织（F=16．391，P〈0．05）。③连续切片染色结果显示，HBD-2蛋白表达水平与CD1a＋DC浸润程度呈正相关（r=0．343，P〈0．05），连续切片结果显示，图片染色的阳性区域相互对应，表示存在位置上的相关性。结论HBD-2在喉鳞癌中的表达与癌细胞的分化程度有关，并受到转录水平和翻译水平上的调节；HBD-2可能通过趋化CD1a＋DC在肿瘤局部的浸润及功能的活化在机体获得性抗肿瘤免疫中发挥作用。

Objective To investigate the expression of human beta-defensin-2 （HBD-2） in laryngeal squamous cell carcinoma （LSCC）, and reveal the immune significance of HBD-2 in LSCC. Methods The expression of HBD-2 and the infiltration of CD1 a ＋dendritic cells were verified by immunohistochemistry strept actividin-biotin complex （SABC） method in thirty-four cases of LSCC＇s paraffin sections, and the expression of HBD-2 mRNA was detected in eighteen cases of postoperative tumor specimens and five cases of normal paracarcinoma tissue using cDNA probe by in situ hybridization. Based on the type and characteristic of datas, SPSS13. 0 program package was used for statistical calculation. Results （1）The difference of expression of HBD-2 was significant among the grades of malignant cell differentiation （ F = 6. 809, P 〈 0. 05 ）, the expression of HBD-2 in well differentiated group was significantly increased compared with moderately and poorly differentiated group （ P 〈 0. 05 ）, there wasn＇t significant difference with the clinical type, T status and lymphatic metastasis in the expression of HBD-2. （2）The difference of expression of HBD-2 mRNA among different grades of malignant cell differentiation was significant （F = 16. 391, P 〈0. 05 ） , less or no positive signal was observed in poorly differentiated group. （3）The infiltration of CD1 a＋ dendritic cells had positive correlation with the expression of HBD-2 （r = 0. 343, P 〈 0. 05）. Conclusions The expression of HBD-2 was related to the grade of malignant cell differentiation in I-SCC, which was regulated at the level of transcription and translation. HBD-2 might play a role in antitumor immunity by chemo-attractiving and activating of immature dendritic cells.