鼻咽癌放疗残灶中HSP70/HSP27蛋白的表达

Expression of HSP70/HSP27 protein in residual lesion after NPC radiotherapy

目的:分析鼻咽癌原发组织HSP70/HSP27蛋白表达与放疗残灶的相关性,探讨HSP70/HSP27表达对鼻咽癌放疗残灶的预测价值。方法:应用SP免疫组织化学法检测58例鼻咽癌原发组织HSP70/HSP27热休克蛋白表达,其中规范放疗后有残存癌灶的28例鼻咽癌为实验组,随访5年无癌灶复发的30例为对照病例。结果:HSP70和HSP27在实验组阳性表达率分别为92.9%(26/28)和53.6%(15/28),而在对照组的阳性表达率为53.3%(16/30)和53.3%(16/30),HSP70阳性表达率在两组间比较有统计学差异(P<0.05),而HSP27阳性表达率在两组间比较无统计学差异(P>0.05);HSP70和HSP27在实验组共同阳性表达率为50.0%(14/28),对照组为16.7%(5/30),两者比较有统计学差异(P<0.05);两者共同阴性表达率在实验组和对照组分别为3.6%(1/28)和10.0%(3/30),两者相比无统计学差异(P>0.05)。结论:HSP70在鼻咽癌抗放射损伤中可能起重要作用,检测鼻咽癌组织HSP70蛋白表达对预测鼻咽癌放射后癌灶残存可能有重要价值。

Objective To analyze HSP70/HSP27 protein expression in the nasopharyngeal carcinoma （ NPC ） primary tissues and the residual lesion, and to explore its predictive value. Methods Immunohistochemical experiment was performed to detect the expression of HSP70 and HSP27 in 58 NPC primary tissues： 28 were in the experimental group with the local residual lesion and 30 in the control group without recurring and metastasis within 5 years by conventional radiotherapy. Results The positive expression of HSP70 and HSP27 in the experimental group was 92.9% （26/ 28） and 53.6% （15/28）, while that in the control group was 53. 3% （16/30） and 53. 3% （16/30） ,respectively. There was significant difference in the 2 groups. The common positive expression of HSP70 and HSP27 between the 2 groups had significant difference, 50.0% （ 14/28 ） in the experimental group and 16.7% （5/30） in the control group, respectively. There was no significant difference in the negative ratio of HSP70 and HSP27 common expression between the 2 groups, 3.6% （1/28） in the experimental group and 10.0% （3/30） in the control group, respectively. Conclusion HSP70 may have an important role in the radioresistanee of NPC, and may predict the residual lesion after radiotherapy.