观察副肿瘤神经综合征患者抗神经抗体(抗Yo抗体)对培养胎鼠神经细胞的影响

The Observation of Impact of Anti-Yo Antibodies in Patients with Paraneoplastie Neurological Syndrome on Fetal Rats'Cultured Neurons

目的观察副肿瘤神经综合征(paraneoplastic neurological syndrome,PNS)患者血清抗神经抗体(抗Yo抗体)对培养胎鼠神经细胞的影响,探讨其免疫学的发病机制。方法实验组PNS患者抗Yo抗体阳性血清IgG、对照组血清IgG,添加到由胎儿鼠脑分离出的培养神经细胞,观察细胞形态变化及细胞凋亡的有无。对照组血清IgG为①AB型健康人标准血清;②无神经损害症状的抗Yo抗体阴性癌症患者血清。结果添加抗Yo抗体阳性IgG的培养神经细胞互相融合,出现粗而长的突起相连的形态变化;未发现凋亡的神经细胞;细胞的边缘被细胞黏附因子N-Cadherin染色。结论 PNS患者血清抗Yo抗体直接作用于胎鼠神经细胞,未发现神经细胞的凋亡,而发现了神经细胞黏附因子的表达,由此推论PNS免疫学发病机制,可能不是抗体与抗原直接作用的结果,而是在血清中存在着抗体以外的某种物质参与了免疫反应。

Objective The impact of the anti - Yo autoantibodies in patients with paraneoplastic neurological syndrome on fetal rats＇ cultured neurons was observed. The etiology of PNS immunopathogenesis was discussed. Methods Serum IgG fraction from anti - Yo antibody positive PNS patient was added to mouse - brain - derived cultured neurons in observation group. Serum from the AB type health person and anti - Yo antibody negative patients without neurological symptoms was used in control group. Transmutations of cell and apoptotic neurons were observed in experiments. Results Cell fusion and conjugation with long and thick dendrites were observed in Yo - IgG observation group. No apoptotic neurons were found and cell surface was stained by the cell adhesion factor of N - cadherin. Conclusion Anti - Yo antibody did not cause neuron apoptosis but induced the expression of N - cadherin in neurons of fetal rats with anti - Yo antibodies serum from patients with paraneoplastic neurological syndrome. It suggests that there is no immunoreaction between antigen and specific antibody, but an immunoreaction by certain and different factors contained in serum IgG fraction in the immunological patho-genesis of PNS.