超声波法提取1-脱氧野尻霉素

Study on Ultrasonic Extraction of 1-Deoxynojirimycin (DNJ) from Mulberry Leaves

研究了利用超声波强化法从桑叶中提取1-脱氧野尻霉素(DNJ)。以乙醇溶液为浸提剂,超声波强化为辅助条件,提取了桑叶中的DNJ。考察了超声功率、料液配比、超声温度以及超声时间等因素对DNJ提取率的影响。结果表明:提取DNJ的最佳条件是超声功率125 W,每克桑叶浸提剂用量40 mL,超声温度70℃,超声时间20 min,DNJ的得率为0.091%,固形物中DNJ纯度为27.2%。

In this study, an ultrasonic--assisted extraction method was developed to extract 1- deoxynojirimycin （DNJ） from mulberry leaves and the process parameters of extraction were optimized by single factor experiment and orthogonal experiment, and acquired as follows： the ultrasonic power of 125 W, solid to water ratio of 1 ： 40, the extraction temperature of 70 ℃, and the extraction time of 20 min. with the optimum conditions, The yield and purity of DNJ was achieved at 0. 091% and 27.2%.