八种蔬菜粉脂溶性粗提物对人胃腺癌细胞增殖的影响

EFFECTS OF THE FAT-SOLUBLE EXTRACTS OF 8 KINDS OF VEGETABLE POWDERS ON THE PROLIFERATION OF HUMAN GASTRIC CANCER CELL

本实验以体外培养的人胃腺癌细胞株SGC－7901为材料，研究了八种蔬菜粉脂溶性粗提物（VPFE）对人胃癌细胞体外增殖的影响。VPEE的剂量（以培养液中卜胡萝卜素浓度计）为2×10^(－8)、2×10^(-7)、2×10^(-6)mol／L。结果：（1）生长曲线实验中，八种菜粉VPFE对胃癌细胞的增殖均有抑制作用。在VPFE高剂量组，各种菜粉的抑制作用均有显著性意义（P＜0.05），而中剂量组的作用在不同菜粉有一些差异；（2）集落形成实验中，冬寒菜、菠菜和紫油菜粉VPFE各剂量组均抑制了胃癌细胞的集落形成（P＜0．01），抑制作用呈剂量一反应关系。本次研究的结果为蔬菜防癌抗癌的实际运用积累资料。

Effects of the fat-soluble extracts of 8 kinds of vegetable powders(VPFE) on the proliferation of human gastric adenocarcinoma SGC-7901 cell line were examined in the present study. The doses of VPFEs were expressed as the level of β-carotene in medium as follows: containing β-carotene 2×10^(-8), 2×10^(-7), 2-10^(-6)mol/L. The results indicated: (1) In the proliferation curve analysis, all of 8 VPFEs showed inhibitory effects on the proliferation of gastric cancer cell line(P＜0. 05 ); (2) In the colony forming assay, 3 VPFEs inhibited the colony forming of gastric cancer cell line in a dose-response manner(P＜0. 01 ).