两株人肺癌细胞摄取^(99)Tc^m-MIBI的比较

Comparison of the Uptake of ^(99)Tc^m-MIBI in Two Lung Cancer Cell Lines

比较2株人肺癌细胞对亲肿瘤显像剂99Tcm-MIBI的不同摄取特征。细胞摄取99Tcm-MIBI的动力学行为显示99Tcm-MIBI在小细胞肺癌(H446)中的摄取显著高于肺腺癌(SPC-1),H446细胞对99Tcm-MIBI的摄取在120 min内逐渐升高至最大峰值,然后缓慢下降;此摄取可被未标记的MIBI所抑制;H446细胞对99Tcm-MIBI的摄取与细胞数量呈正相关而与放射性浓度呈负相关;SPC-1细胞对99Tcm-MIBI的摄取处于低水平状态,无明显峰值。结果显示,不同的肺癌细胞具有对99Tcm-MIBI的不同摄取特性,这与临床不同类型肺癌患者的99Tcm-MIBI显像结果不同相符。

The uptake characters of ^99Tc^m-MIBI in two lung cancer cell lines （human small cell lung cancer H446 cells and human lung adenocarcinoma SPC-1 cells） were investigated. The uptake kinetic data show that H446 cells absorb significantly higher level of ^99Tc^m-MIBI comparing with SPC-1 cells. The uptake percentage of ^99Tc^m-MIBI in H446 cells increases time-dependently and reaches its peak value at about 120 min, and then droppes slowly. This uptake in H446 cells is positively correlated with the number of cells while negatively with the concentration of radiolabelled 99Tcm_MIBI. Furthermore, it is markedly inhibited by excessive unlabelled MIBI. However, the uptake of ^99Tc^m-MIBI in SPC-1 cells maintains at low levels without a visible peak during the experiment time. These results suggest that the uptake characters of ^99Tc^m-MIBI are different in two lung cancer cell lines, which is accordant with the clinical facts that patients with lung cancers displayed diverse ^99Tc^m-MIBI imaging patterns.