基于二氧化锆和碳纳米管协同作用的过氧化氢生物传感器

Study on hydrogen peroxide biosensor based on the synergy between zirconiu dioxide and carbon nanotubes modified glass carbon electrode

A novel hydrogen peroxide(H2O2)biosensor was prepared.First,zirconium dioxide(ZrO2)were solubilized in the aqueous solution of a biopolymer chitosan(CS)and the mutlicarbon nanotubes(MWNTs)was introduced into the ZrO2-CS solution to get a ZrO2-CS-MWNTs composite.Then the ZrO2-CS-MWNTs composite was immobilized onto the surface of the glass carbon electrode(GCE).Subquently,a stable nano-Au film was electrodeposited on the ZrO2-CS-MWNTs modified electrode at a constant potential.Finally,horseradish peroxidase(HRP)was immobilized onto the nano-Au layer to obtain the HRP/nano-Au/ ZrO2-CS-MWNTs /GCE electrode.The results showed that the biosensor exhibited well reduction ability for hydrogen peroxide with high sensitivity and fastresponse time resulting from.The biosensor displayed a rapid response to H2O2 and the linear range of the biosensor was from 7.80×10-6 to 9.26×10-3 mol/L with the detection limit of 2.8×10-6 mol/L(S/N=3).In addition,the biosensor showed fast response,high sensitivity,good stability and reproducibility.

A novel hydrogen peroxide （ H2O2 ） biosensor was prepared. First, zirconium dioxide （ ZrO2 ） were solubilized in the aqueous solution of a biopolymer chitosan （CS）and the mutliearbon nanotubes （MWNTs）was introduced into the ZrOz-CS solution to get a ZrO2-CS-MWNTs composite. Then the ZrO2-CS-MWNTs composite was immobilized onto the surface of the glass carbon electrode （GCE）. Subquently,a stable nano-Au film was eleetrodeposited on the ZrO2-CS-MWNTs modified electrode at a constant potential. Finally,horseradish peroxidase （HRP）was immobilized onto the nano-Au layer to obtain the HRP/nano-Au/ ZrO2-CS- MWNTs/GCE electrode. The results showed that the biosensor exhibited well reduction ability for hydrogen peroxide with high sensitivity and fastresponse time resulting from. The biosensor displayed a rapid response to H2O2 and the linear range of the biosensor was from 7.80 × 10^ -6 to 9.26 × 10 ^-3 mol/L with the detection limit of 2.8 × 10 -6mol/L（ S/N = 3 ）. In addition,the biosensor showed fast response, high sensitivity, good stability and reproducibility.