不同产地广佛手药材HPLC指纹图谱的研究

HPLC fingerprint of Fructus Citri Sarcodactylis from Guangdong province

目的:采用高效液相色谱法建立广佛手药材的指纹图谱,为其品质控制提供依据。方法:采用HPLC分析广佛手的指纹图谱。色谱柱:Diamonsil C18(250mm×4.6mm,5μm);流动相:甲醇-0.3%乙酸溶液梯度洗脱;分析时间:60min;检测波长:283nm;柱温:25℃;流速:1.0mL/min。结果:建立了广佛手的指纹图谱,确定了13个共有峰。结论:该指纹图谱检测方法简便、重现性好,可作为广佛手药材质量控制的重要方法。

AIM：To establish chromatographic fingerprint of Fructus Citri Sarcodactylis from Guangdong province by HPLC and it was expected to fix standard of quality control. METHODS：Analysis was performed on Diamonsil C 18 column （250 mm×4.6 mm,5 μm） with gradient mobile phase of methanol-0.3% acetic acid,fingerprint was finished in 60 min,the monitoring wavelength was at 283 nm and the column temperature was at 25 ℃ with the flow rate of 1.0 mL/min. RESULTS：Establishing the fingerprint of Fructus Citri Sarcodactylis from Guangdong province, 13 common peaks were found in the HPLC fingerprints from different sources. CONCLUSION： This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Fructus Citri Sarcodactylis from Guangdong province.