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RP-HPLC同时测定三黄片中盐酸小檗碱、盐酸巴马汀、土大黄苷含量 被引量:6

Simultaneous determination of berberine hydrochloride,palmatine hydrochloride and rhaponticin in Sanhuang Tablets by RP-HPLC
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摘要 目的:用RP-HPLC法同时测定三黄片(大黄、盐酸小檗碱、黄芩浸膏)中盐酸小檗碱、盐酸巴马汀、土大黄苷的含量。方法:色谱柱:Shimadzu色谱柱(250mm×4.6mm,5μm);流动相为乙腈-0.05%十二烷基硫酸钠(含0.1%磷酸),梯度洗脱;流速:1.0mL/min;检测波长:334nm,柱温:25℃。结果:盐酸小檗碱、盐酸巴马汀、土大黄苷分别在0.05~1.04μg(r=0.9999),0.02~0.49μg(r=0.9998),0.05~1.02μg(r=0.9999),范围内线性关系良好;3个成分的平均回收率(n=6)分别为98.7%,97.2%,99.3%。结论:本方法准确可靠,适用于三黄片的质量控制。 AIM:To determine the contents of berberine hydrochloride,palmatine hydrochloride and rhaponticin in Sanhuang Tablets(Radix et Rhizoma Rhei,berberine hydrochloride,extract of Radix Scutellariae) by RP-HPLC. METHODS:RP-HPLC method with Shimadzu TM column(250 mm×4.6 mm,5 μm) was used. The gradient elution mixture mobile phase consisted of acetonitrile and 0.05% Lauryl sodium sulfate(0.1% phosphoric acid). The flow rate was 1.0 mL/min. The detection wavelength was set at 334 nm. The temperature of column was at 25 ℃. RESULTS: The calibration curves of berberine hydrochloride, palmatine hydrochloride and rhaponticin were linear within the ranges of 0.05 - 1.04 μg( r = 0.999 9 ), 0.02 - 0.49 μg( r = 0. 999 8 ) ,0.05 - 1.02 μg( r = 0. 999 9 ), respectively. The average recoveries (n = 6 ) were 98.7%, 97.2%, 99.3%, respectively. CONCLUSION: The method of the operation is accurate, reliable and can be used to control the quality of Sanhuang Tablets.
作者 彭乃焕
出处 《中成药》 CAS CSCD 北大核心 2009年第12期1860-1862,共3页 Chinese Traditional Patent Medicine
关键词 三黄片 盐酸小檗碱 盐酸巴马汀 土大黄苷 HPLC Sanhuang Tablets berberine hydrochloride palmatine hydrochloride rhaponticin RP-HPLC
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