RP-HPLC同时测定三黄片中盐酸小檗碱、盐酸巴马汀、土大黄苷含量

Simultaneous determination of berberine hydrochloride,palmatine hydrochloride and rhaponticin in Sanhuang Tablets by RP-HPLC

目的:用RP-HPLC法同时测定三黄片(大黄、盐酸小檗碱、黄芩浸膏)中盐酸小檗碱、盐酸巴马汀、土大黄苷的含量。方法:色谱柱:Shimadzu色谱柱(250mm×4.6mm,5μm);流动相为乙腈-0.05%十二烷基硫酸钠(含0.1%磷酸),梯度洗脱;流速:1.0mL/min;检测波长:334nm,柱温:25℃。结果:盐酸小檗碱、盐酸巴马汀、土大黄苷分别在0.05～1.04μg(r=0.9999),0.02～0.49μg(r=0.9998),0.05～1.02μg(r=0.9999),范围内线性关系良好;3个成分的平均回收率(n=6)分别为98.7%,97.2%,99.3%。结论:本方法准确可靠,适用于三黄片的质量控制。

AIM：To determine the contents of berberine hydrochloride,palmatine hydrochloride and rhaponticin in Sanhuang Tablets（Radix et Rhizoma Rhei,berberine hydrochloride,extract of Radix Scutellariae） by RP-HPLC. METHODS：RP-HPLC method with Shimadzu TM column（250 mm×4.6 mm,5 μm） was used. The gradient elution mixture mobile phase consisted of acetonitrile and 0.05% Lauryl sodium sulfate（0.1% phosphoric acid）. The flow rate was 1.0 mL/min. The detection wavelength was set at 334 nm. The temperature of column was at 25 ℃. RESULTS： The calibration curves of berberine hydrochloride, palmatine hydrochloride and rhaponticin were linear within the ranges of 0.05 - 1.04 μg（ r = 0.999 9 ）, 0.02 - 0.49 μg（ r = 0. 999 8 ） ,0.05 - 1.02 μg（ r = 0. 999 9 ）, respectively. The average recoveries （n = 6 ） were 98.7%, 97.2%, 99.3%, respectively. CONCLUSION： The method of the operation is accurate, reliable and can be used to control the quality of Sanhuang Tablets.