毛细管气相色谱法同时测定黑鬼豆油中6种成分的含量

Determination of six components in Heiguidou Oil by capillary GC

目的:建立黑鬼豆油中有效成分α-蒎烯、桉油精、龙脑、水杨酸甲酯、桂皮醛、丁香酚的气相色谱测定法。方法:采用气相色谱法,色谱条件以PEG-20M毛细管柱(30m×0.25mm×0.25μm),FID检测器,程序升温,起始温度80℃,保持7min后每分钟升15℃,终止温度180℃,保持10min,分流比50∶1。样品用无水乙醇溶解,采用外标法定量。结果:α-蒎烯的线性范围0.322～32.150mg/mL,桉油精的线性范围0.202～20.215mg/mL,龙脑的线性范围0.126～12.630mg/mL,水杨酸甲酯的线性范围0.633～63.290mg/mL,桂皮醛的线性范围0.174～17.390mg/mL,丁香酚的线性范围0.203～20.255mg/mL。RSD分别为0.83%、0.48%、0.48%、0.31%、0.53%。结论:方法简便、快速、准确,能同时测定该产品6种成分的含量。

AIM：To develop a capillary GC method for determining six components in Heiguidou Oil,α-pinene,eucalyptol,borneol,methyl salicylate,cinnamia aldehyde and eugenol. METHODS：GC was applied to quantitative analysis. Chromatographic conditions consisted of PEG-20M（30 m×0.25 mm×0.25 μm）,flame ionization detector（FID）,and programmed temperature; initiation temperature was at 80 ℃ for 7 min; the rate of temperature was 15℃/minute,final temperature was at 180 ℃ for 10 min,bypass rate was 50 ∶1. The sample dissolved in absolute alcohol. The external standard was used. RESULTS： The standard curves of conclusion α-pinene,eucalyptol,borneol,methyl salicylate,einnamia aldehyde and eugenol were linear within the ranges of 0. 322 -32. 150 mg/mL,0. 202 - 20. 215 mg/mL,0. 126 - 12. 630 mg/mL,0. 633 - 63. 290 mg/mL,0. 174 - 17. 390 mg/mL, 0. 633 - 63. 290 mg/mL and 0. 203 - 20. 255 mg/mL, respectively. RSD were 0.83% ,0.48% ,0.48% ,0.31%, and 0.53% ,respectively. CONCLUSION： The established method is accurate and it can be applied to the simulta-neous assay of six components.