大鼠肾阳虚证的代谢组学研究

Metabonomics Study on Kidney-Yang Insufficiency Rats

目的利用代谢组学技术观察糖皮质激素所造的肾阳虚证大鼠模型的内源性代谢物特征。方法将24只SD雄性大鼠随机分为正常组、模型组和模型给药组各8只；肌注氢化可的松琥珀酸钠造模；造模成功后，给予模型给药组大鼠白附片溶液灌胃，连续14d；末次给药24h后取血制备血浆；采用高效液相色谱一质谱联用的代谢组学技术检测内源性代谢物。结果正常组与模型组相比，溶血磷脂酰乙醇胺（LPE）、溶血磷脂酰胆碱（LPC）和468．4m／z的未知化合物的谱峰峰形改变较为明显。发生改变的代谢物可作为肾阳虚证的生物标志物做进一步研究。结论从代谢组学分析中找出特异的标志性代谢产物，以利于深入研究中医证候的生物学本质。

Objective To explore the endogenesis metabolizer character of kidney-yang insufficiency syndrome model rats caused by hydrocortisoni natrii succinas with metabolomics technology. Methods Twenty four Sprague-Dawley （SD）male rats were divided randomly into a normal group, a kidney-yang insufficiency model group, and a oral administration group, eight rats in each group. After producing kidney-yang insufficiency model by injecting hydrocortisoni natrii succinas intramuscularly, oral administration group rats were administered orally with white prepared lateral root of aconite every day for two weeks. After twenty-four hour the last oral administration, the blood plasm were prepared and used for testing endogenesis metabolism with the liquid phase color spectrum-mass spectra（LC/MS） metabolomics technology. Results Apices shape change of lysophosphatidyl choline （LPC） and lysophosphatidyl ethanolamine （LPE） and 468.4m/z unbeknown chemical compound of normal rats were distinct from those of model rats. Above-mentioned chemical compound as chief material symbols of kidney-yang insufficiency syndrome might be farther studied. Conclusion Finding out differential symbol from endogenesis metabolizer with metabolomics technology was redounded to deep exploring the biology essence of traditional Chinese medicine syndrome.