槲皮素金属配位分子印迹聚合物的识别性能

Molecular Recognition Characteristics of Quercetin's Metal-complexing Imprinted Polymer

以槲皮素与Zn(Ⅱ)的配合物为模板,在甲醇溶液中制备金属配位分子印迹聚合物.通过紫外光谱研究了槲皮素与Zn(Ⅱ)的配位方式及配位比,验证了槲皮素、Zn(Ⅱ)和4-乙烯基吡啶之间的三元配位作用.利用红外光谱对产物的结构进行了表征.用平衡结合实验考察了功能单体及交联剂用量对聚合物吸附性能的影响,优化了聚合物的反应配比.同时对系列印迹聚合物的识别体系进行了考察.结果表明,槲皮素-Zn(Ⅱ)模板印迹聚合物对槲皮素-Zn(Ⅱ)的配合物表现出明显的吸附选择性和特异性,对槲皮素结构类似物芦丁和柚皮素的吸附选择性较差,分离因子分别为3.21和1.91.

Using Zn（Ⅱ）-quercetin complex as template, a metal-complexing template polymer was prepared in methanol by molecular imprinting technique. The coordination mode and complex molar ratio between quercetin and Zn（Ⅱ） were studied in differential UV absorption spectrometry, and the ternary complexation of quercetin, Zn（Ⅱ） and 4-vinylpyridine was verified by similar methods. The molecularly imprinted polymers（MIPs） prepared were analyzed via infrared spectra, and it showed that MIPs had the necessary characteristics of the structure. In order to optimize the reaction ratio of MIPs, the effect of functional monomer and cross-linking agent dosage on the binding capacity of MIPs was studied with equilibrium binding experiments. The selective binding experiments for substrates indicated that compared with quercetin′s （rutin and naringenin）, Zn（Ⅱ）-quercetin template polymer exhibited significant adsorption selectivity for Zn（Ⅱ）-quercetin complex. The separation factors were 3.21 and 1.91 respectively.