中药抑肝纤和促肝细胞生长素对大鼠免疫性肝纤维化的阻断作用

Blocking effect of Chinese herbs Yiganxian and PHGF on immuno-damaged hepatic fibrosis in rats

目的用人血清清蛋白免疫损伤Ｗｉｓｔａｒ大鼠造成肝纤维化模型，观察中药抑肝纤和促肝细胞生长素对大鼠肝纤维化的阻断作用．方法将不同浓度（含生药１２ｋｇ／Ｌ或２４ｋｇ／Ｌ两种）中药抑肝纤（主要含黄芪，丹参，鳖甲，牡蛎等）和促肝细胞生长素用于治疗组并与模型组和对照组进行对比，综合分析血清肝纤维化指标（ＰＣⅢ，ＨＡ，ＬＮ）、肝组织病理改变、肝组织胶原纤维和网状纤维病理图象定量分析等结果．结果治疗组血清肝纤维化指标ＰＣⅢ（μｇ／Ｌ；１２ｋｇ／Ｌ组为９２４±２５２ｖｓ１５９６±３７０，Ｐ＜００１）ＨＡ（μｇ／Ｌ；１２ｋｇ／Ｌ组为２４３７±１０６８ｖｓ４８１２±１２１１，Ｐ＜００１）ＬＮ（μｇ／Ｌ；１２ｋｇ／Ｌ组为８８９±２６４ｖｓ１４２３±５４４，Ｐ＜００１）均低于模型组，接近正常组（三项指标分别为９１６±３２６，２３６６±１２１２及９２５±２３５）差异有显著性．光镜下肝组织病理变化，肝组织中胶原纤维和网状纤维病理图象定量分析结果治疗组优于模型组，尤以中药低浓度组更为明显（Ｐ＜００１或Ｐ＜００５）．结论研究结果表明，中药抑肝纤和促肝细胞生长素如能早期应用，可有效地阻?

IM To study the blocking effects of traditional Chinese herbs Yiganxian and promoting hepatocyte growth factor (PHGF) on immunodamaged liver fibrosis in rats.METHODS Abstract:Treatment groups with various doses (including dried medicinal herbs (12kg/L or 24kg/L) traditional Chinese herbs Yiganxian and PHGF were compared with model group and control group. Serum PCⅢ, HA and LN, pathological changes of hepatic tissues, and results of collagenous fibers and reticular fibers pathological picture of the liver were analyzed.RESULTS Serum PCⅢ (μg/L; in the 12kg/L group 924±252 vs 1596±370,P<001) HA (μg/L; 2437±1068 vs 4812±1211,P<001) LN (μg/L; 889±264 vs 1423±544,P<001) in the treatment group were obviously lower than model group,and close to control group (μg/L; 916±326, μg/L 2366±1212, μg/L; 925±235). Pathological results under microscope and results of collagenous and reticular fibers pathological picture in the liver were better than model group especially in the group of lowdose traditional Chinese herbs (P<001/P<005).CONCLUSION Early administration of traditional Chinese herbs Yiganxian and PHGF can effectively prevent liver fibrosis in rats.