胆结石与胆道幽门螺旋杆菌的相关性研究

The relationship of Helicobacter pylori and cholecystolithiasis

目的探讨胆道幽门螺杆菌与胆结石的相关性。方法收集38例胆结石患者的胆囊粘膜、胆汁、结石标本(实验组)和20例非结石患者的胆囊粘膜、胆汁标本(对照组)。采用酶联免疫吸附试验(ELISA)、免疫组织化学技术分别对两组患者的胆汁、结石和胆囊粘膜中的幽门螺旋杆菌进行检测。结果实验组胆囊粘膜、胆汁、结石幽门螺杆菌检测阳性率分别为15.9%、81.2%、60.0%;对照组胆囊粘膜、胆汁幽门螺杆菌检测阳性率分别为25.0%、52.9%;实验组与对照组之间胆汁幽门螺杆菌阳性率分别为81.2%、52.9%,差异有统计学意义。结论胆结石患者胆道系统中存在幽门螺杆菌感染,幽门螺杆菌可能与胆结石的形成有关。

Objective To investigate the relationship of Helicobacter pylori and cholecystolithiasis. Methods ELISA and immunohistochemistry approach were used to detect Helicobacter pylori from gallstone,bile and gallbladder mucosa in 38 cholecystolithiasis patients （experimental group）and 20 patients without gallstone （control group）. Results In experimental group,the positive rate of Helicobacter pylori in gallbladder mucosa,bile and gallstone was 15.9%, 81.2%, 60.0%.In control group,the positive rate of Helicobacter pylori in gallbladder mucosa and bile was 25.0%, 52.9%. Cholecystolithiasis patients compared with patients without gallstone ,the positive rate of Helicobacter pylori in bile was 81.2%,52.9%,there was significant difference （P〈0.05）. Conclusion Helicobacter pylori are commonly present in the cholecystolithiasis of patients,and maybe the Helicobacter pylori play a role in the formation of gallstones.