庆大霉素对豚鼠耳蜗ACP的影响

THE EFFECTS OF GENTAMICIN ON ACID PHOSPHATASEIN GUINEA PIG'S COCHLEA

目的观察庆大霉素对豚鼠耳蜗ＡＣＰ的影响。方法以耳廓反射及酸性磷酸酶（ＡＣＰ）为指标、观察正常及庆大霉素耳中毒豚鼠的耳蜗组织。结果正常豚鼠耳蜗内ＡＣＰ主要集中在耳蜗的毛细胞内，并显示出毛细胞的排列形式；庆大霉素耳中毒的豚鼠耳蜗毛细胞内ＡＣＰ出现位移、显色变淡或消失。耳廓反射阈值增加越明显者，ＡＣＰ变化越显著。结论实验表明庆大霉素引起的耳蜗毛细胞的损坏与耳蜗毛细胞内溶酶体膜破裂释放出的ＡＣＰ等水解酶的自溶性损坏有关。

Objective To observe the effects of GENTAMICIN on acid phosphatase in guinea pig's cochlea. Methods From normal and gentamicin induced ototoxic guinea pig's cochlear tissues, the variations of auricula reflex and acid phosphatase were observed. Results Acid phosphatase mainly existed in hair cells in normal guinea pig's cochlear tissues, and the arrangement of hair cells was shown; Acid phosphatase in gentamicin induced ototoxic guinea pig's cochlear tissues was found displaced and poor colored, The more remarkally the threshold increased,the more remarkally acid phosphatas changed. Conclusion This experiment suggests gentamicin may induce the release of acid phosphatase by damaging the lysosome's membranein cochlear hair cells.