B7-1基因转染小鼠EL-4淋巴瘤细胞的致瘤性研究

STUDY ON ONCOGENICITY OF RAT EL -4 LYMPHOMA CELL TRANSFECTED WITH GENE B7-1

目的研究Ｂ７１基因转染小鼠ＥＬ４淋巴瘤细胞的致瘤性，为制备基因转染瘤苗提供实验依据。方法将Ｂ７１基因转染小鼠ＥＬ４淋巴瘤细胞后，用Ｇ４１８选择和克隆的方法获得高表达Ｂ７１分子的ＥＬ４Ｂ７１＋细胞，然后进行小鼠的体内致瘤试验。结果ＥＬ４Ｂ７１＋细胞接种小鼠后，与野生型ＥＬ４（ＥＬ４Ｗｔ）相比，肿瘤结节形成时间和荷瘤小鼠存活时间明显延长，ＥＬ４Ｂ７１＋细胞协同ＩＬ２接种小鼠后，效果更加显著。结论Ｂ７１基因转染小鼠ＥＬ４淋巴瘤细胞的致瘤性比野生型ＥＬ４（ＥＬ４Ｗｔ）低。

Objective To study the Oncogenicity of rat EL4 lymphoma cell transfected with Gene B71 and to provide experimental basis for preparing tumor vaccine by transfected gene.Method oncogenic experiment in vivo was carried out after EL4B71+ cell,which was the high expressioon of B71 molecule,was obtained by G418 after rat EL4 lymphoma cell being transfected with gene B71.Result Compared with wild type EL4(EL4Wt),the formation time of tumor nodes and survival time of rats with tumor were remarkably prolonged.The result was more significant when EL4B71+ and IL2 were used together. Conclusion The oncogenicity of rat EL4 lymphoma cell transfected with gene B71 is lower than that of wild type EL4 lymphoma cell