硫代乙酰胺诱导大鼠肝纤维化的研究

Study on rat liver fibrosis induced by thioacetamide

目的研究硫代乙酰胺对大鼠肝纤维化模型的诱导作用。方法采用0.03%的硫代乙酰胺腹腔注射,每周2次,构建大鼠肝纤维化模型;观察大鼠肝纤维化形成情况。结果与空白组比较,模型组大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、透明质酸(HA)明显升高(P<0.01);层粘连蛋白(LN)升高(P<0.05)。组织病理提示模型组大鼠肝细胞大量坏死,假小叶形成。结论硫代乙酰胺能诱导大鼠形成肝纤维化。

Objective To study on rat liver fibrosis model induced by thioacetamide. Methods With 0.03% thioacetamide intraperitoneally two times a week ,to build model of liver fibrosis and to observe the formation of rat liver fibrosis.Results Compared with the control group, the levels of rat serum AST, ALT and HA in model group increased significantly higher （P〈0.01） and the level of rat serum LN in model group increased higher （P〈 0.05） . Histopathology of rat liver tissue in model group prompted a large number of liver cells necrosis and the formation of pseudo-lobular. Rats in model group formed liver fibrosis.Conclusion Thioacetamide can induce the formation of liver fibrosis in rats.