柱前衍生高效液相色谱测定小儿复方氨基酸注射液中氨基酸

Determination of Amino Acids in Children Compound Amino Acid Injection by Pre-column Derivatization High Performance Liquid Chromatography

建立了高效液相色谱（HPLC）测定小儿复方氨基酸注射液中各种氨基酸含量的方法。采用Kromasil C18（250×4．6mm，5μm）色谱柱，以2，4-二硝基氟苯为衍生试剂，梯度洗脱。流动相A为乙腈：水（50：50，V／V），流动相B为0．04mol／L的磷酸二氢钾溶液（pH=6．8）；流速为1．0mL／min；检测波长为360nm。所测氨基酸的线性范围为0．008-0．2213mg／mL，平均回收率在97．85％～102．86％之间。

A pre-column derivatization high performance liquid chromatogrphy method for the determination of amino acids in the children compound amino acid injection was established. The sample was derivatized with 2,4-dinitrofluorobenzene（DNFB）. Kromasil C18 column （250 × 4.6 mm, 5μm） was used. The gradient elution was performed using mixed solution of acetonitrile and water （50：50,V/V）, and 0.04 mol/L KH2PO4 （pH=6.8） as the mobile phases respectively at a flow rate of 1.0 mL/min. All the amino acids measured show good linear relationship within 0. 008 - 0. 2213 mg/mL and average recoveries between 97.85% and 102.86%.