银杏叶提取物与刺梨配伍抗实验性肝损伤作用

Effects of compatibility program of Ginkgo biloba extracts and Rosa roxburghii on experimental liver injury

目的:比较复方银杏叶胶囊(CGB)与单方银杏叶提取物(GBE)的保肝作用。方法:用CGB、GBE给小鼠灌胃30 d后,腹腔注射CCl4诱发肝损伤,测定小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及肝匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)含量;用H2O2诱导人肝L-02细胞氧化损伤,观察细胞增殖及总抗氧化能力。结果:CGB 2.4、0.8和0.4 g/kg3个剂量组与模型组比较,小鼠血清ALT、AST明显降低(P<0.01,P<0.05),同时肝匀浆中SOD显著升高、MDA下降(P<0.01,P<0.05);CGB组细胞增殖率和总抗氧化能力分别较模型组和单方组显著提高(P<0.05、P<0.01)。结论:CGB的抗肝损伤作用优于单方银杏叶提取物。

Objective：To study the protective effect of compound Ginkgo biloba （CGB） on hepatic injury. Methods： Animal model of chemical hepatic injury was established by ip CCl4 in mice, the content of ALT, AST in rats＇serum, the content of MDA and the activity of SOD in liver homogenate were assayed after intragastric administration 30 days in mices with Ginkgo biloba extracts（ GBE）, compound ginkgo biloba （CGB）. The model of human normal hepatocyte L-02 induced by H2O2 was prepared, determining the level of total antioxidant capacity in serum （TAOC） , and the proliferation of hepatoeyte L-02 using MTT. Results： The content of AST, ALT in rats＇serum levels in groups of CGB （2.4,0.8 g/kg） decreased significantly（ P 〈 0. 01, P 〈 0.05 ）, the activity of SOD increased singnificantly and the MDA levels decreased obviously in liver homogenate of mices. The CGB can remarkably increase the level of TAOC and the proliferation of liver cell L-02. Conelusion：CGB are better than GBE in protecting chemical liver injury.