不同酶解条件对金边瑞香幼叶原生质体分离的影响

The Effect of Different Decomposing Conditions on Protoplast Isolation of Young Leaves of Daphne odora var.marginata

以室内培养的金边瑞香浅黄绿色、质地幼嫩的叶片为材料,探讨了影响其原生质分离的因素。结果表明:对原生质分离效果影响最大的是纤维素酶浓度和酶解时间;采用纤维素酶质量分数为0.2%、甘露醇质量浓度为0.6 mol.L-1、酶解时间为10 h时原生质分离效果最好,原生质体的产量为2.2×105个.g-1。

The factors which influenced the process of protoplast isolation was researched with buff-green young leaves of Daphne odora var.marginata cultured indoor.The result suggested that cellulose enzyme concentration and decomposing time effected protoplast isolation effect mostly,2.2×10^5 protoplasts·g^-1 was obtained with 0.2%（w/v）of Cellulose R-10,and 0.6 mol·L^-1 of mannitol after 10-hour decomposing