多指标自身抗体联合检测与乙型肝炎DNA关系分析

Relationship between multi-index auto-antibodies and HBV-DNA

目的:探讨自身抗体与乙型肝炎病毒感染的关系。方法:用酶免疫斑点法测定乙型肝炎患者抗心磷脂抗体(ACA)、抗平滑肌抗体(ASMA)、抗核抗体(ANA)。结果:45例乙型肝炎患者中ACA、ASMA、ANA检出阳性率显著高于健康对照组(P<0.05)。实验组自身抗体的产生与HBV-DNA实时荧光定量PCR结果成对应关系的ACA有8例,阳性率为17.78%;AMSA 7例,阳性率为15.56%;ANA 10例,阳性率为22.23%;与肝酶谱ALT、AST、GGT的关系表现为ACA 10例,阳性率为22.23%;AMSA3例,阳性率为6.67%;ANA 12例,阳性率为26.67%;HBV-DNA实时荧光定量PCR对数值梯度与肝酶谱的释放成正比。结论:ACA、ASMA和ANA的产生与是否感染乙型肝炎无关,但与乙型肝炎病毒是否活跃和肝功能的损伤程度有关。

Objective： To analysis the relationship between multi-index antoantibodies and HBV-DNA. Method： Determination of hepatitis B in patients with anticardiolipin antibodies（ACA）, anti-smooth muscle antibodies（ASMA）, anti-nuclear antibody（ANA） by enzyme-linked immunosorbent assay. Results：The positive ratio of ACA, ASMA, ANA-in patients with hepatitis B were significantly higher than that of the healthy control group respectively （P〈0. 05）. The correlation between the emergence of auto-antibodies in experimental group and the results of the HBV-DNA by real-time fluorescence quantitative PCR were found. 9 cases of the positive results of ACA , 7 cases of the positive results of AMSA and the 10 cases of the positive results of ANA were found in 45 case of patients with hepatitis B. The positive ratio were 17.78%, 15.. 56%, 22.23% respectively. The relationship between self-antibodies of the ACA, AMSA, ANA and the liver enzymes were found. 10 case of the ACA（positive rates：22. 3 %）, 3 case of the AMSA（positive rates：6. 67%） and 12 case of the ANA（positive rates： 26. 67%）. The numerical gradient of HBV-DNA by real-time fluorescence quantitative PCR is proportional to the emergence of the self-antibodies and the release of liver enzymes. Conclusions： ACA, ASMA and ANA were related to the injury of liver function.