Cem-Ostetic^(TM)人工骨浆复合骨形态发生蛋白对山羊椎体骨缺损修复作用研究

Repair effects of Cem-Ostetic^(TM) as carrier for bone morphogenetic protein on bone defect of centrum in goat

目的观察人工骨浆复合骨形态发生蛋白(BMP)对成年山羊椎体骨缺损的修复作用,并探讨其临床应用的可行性。方法将健康成年山羊24只随即分成单纯Cem-OsteticTM人工骨浆组(A组)和Cem-OsteticTM/BMP人工骨浆复合骨形态发生蛋白组(B组),每组12只动物。在山羊胸腰段3处不相邻椎体分别建立椎体压缩性骨折撑开复位后骨缺损模型。将Cem-OsteticTM/BMP人工骨浆复合材料填充于缺损处,同时设立单纯人工骨浆对照组。术后4周、8周、12周分别处死动物,每组每次处死4只。通过大体观察,影像学检查,HE染色和生物力学测试。结果术后4周及8周时B组成骨情况、材料降解速度及生物力学强度和刚度测试明显优于A组(﹤0.05),但仍未达到正常椎体的力学性能,差异有显著性(﹤0.01)。第12周时,两组X线及CT下均可见大量新骨生成,基本完整填充空隙,填充材料均基本完全降解,生物力学测试两组强度和刚度无显著性差异,基本达到了正常椎体的力学性能。结论Cem-OsteticTM人工骨浆是BMP的良好载体,Cem-OsteticTM/BMP复合材料具有较强的传导成骨和诱导成骨活性,生成的新骨有良好的强度和刚度。

Objective To observe the repair effects of the artificial bone paste mixed bone morphogenetic protein （BMP） on bone defect ofcentrum in goat and to explore the feasibility of its clinical application. Methods 24 healthy adult goats were divided into group A and group B, 12 animals in each group. The vertebral body model of vertebral compression fractures after open reduction was established respectively on 3 unconnected vertebrae of each goat. Cem-Ostetic^TM/BMP composite material was filled in the gaps, at the same time,a simple artificial paste control group was established. The goats were executed after operation 4 weeks, 8 weeks and 12 weeks, each executed 4 goats each group. Through the general observation of vital signs, imaging examination, HE staining and biomechanical testing to understand the new bone formation and material degradation. Results After operation 4 weeks and 8 weeks ,the ossification, the material degradation, biomechanical strength and stiffness test of group B were better than group A （P 〈0.05）,but it still has not reached the mechanical properties of the normal vertebral body, there was a significant difference （P 〈0.01）.After 12 weeks, a large amount of new bone formation can be seen in the X-ray and CT of two groups, almost filled the gaps.The filled materials almost were degradation, biomechanical strength and stiffness test in the two groups were no significant difference. And it almost reached the mechanical properties of the normal vertebral body. Conclusions Cem-Ostetic^TM artificial bone paste is a good carrier ofBMP, Cem-OsteticTM / BMP composite material has a strong conduction and induced activity to produce new bone. The new bone had a good strength and stiffness.