不饱和聚磷酸酯/β-磷酸三钙复合材料修复骨缺损的实验研究

A Experimental Study on Repairing Bone Defects by Unsaturated Polyphosphoester/βeta-Tricalcium phosphate Composite Material

目的观察以不饱和聚磷酸酯(UPPE)/β-磷酸三钙(β-TCP)制备的复合材料对骨缺损的修复效果。方法利用溶液浇铸法技术制备UPPE/β-TCP复合材料。将36只新西兰大白兔随机等分为两组,手术造成右股骨髁部腔洞状直径8mm骨缺损,实验组植入直径8mm复合材料,对照组作空白对照。通过影像学、组织学、图像分析技术观察骨缺损的修复效果及材料的降解情况。结果影像学结果表明,实验组术后8周新生骨痂将缺损修复,术后16周骨痂塑形良好;对照组术后16周内均无骨痂形成,缺损未修复。组织学显示,实验组术后8周复合材料降解成较大的数块,有新骨形成;术后16周正常骨与材料之间形成贯通的髓腔;对照组为少量纤维组织连接。术后8周和16周材料降解率分别是30.3%和52.2%。结论以不饱和聚磷酸酯/β-磷酸三钙制备的骨修复材料对骨缺损具有良好的修复作用。

Objective To observe the effects of composite material from Unsaturated Polyphosphoester（UPPE） /βeta- Tricalcium phosphate（β-TCP） on repairing bone defects. Methods UPPE/β-TCP composite material were made by solvent casting. 36 New Zealand rabbits were divided into two groups randomly. Operations were done to make a 8 mm bone defect at right femoral condyle in each rabbit. In the experiment group, composite material was implanted into the defect; in the control group, nothing was implanted. The results were evaluated by radiograph,histology, and the degradation rate of the composite material was analyzed by an image analysis system. Results As was shown in radiography, the bone defect was repaired by newly-formed callus in the experiment group by 8 weeks after operation,the callus molded well by16 weeks postoperatively;In the control group, by 16 weeks after operation, no callus formed and the defects were not repaired. Histologically, in the experiment group, by 8 weeks after operation, the composite material degraded into bigger pieces and new bone formed. By 16 weeks, medullary cavity formed between normal bone and composite material; in the control group,only detected some fibrous tissue. 8 weeks postoperatively, the degradation rate of the composite material was 30. 3% in the experiment group, which rose to 52.2% 16 weeks after operation. Conclusion The composite material which was made of UPPE/β-TCP has satisfactory repairing effect on the bone defects.