连翘籽化学成分研究

Studies on the Chemical Constituents from the Seed of Forsythia suspense

目的：研究连翘籽的化学成分。方法：采用乙醇提取,硅胶柱色谱分离纯化,采用理化数据和光谱法鉴定化学成分的结构。结果：共分离得到了7个化合物,分别鉴定为β-香树脂醇乙酸酯（Ⅰ）、20（S）-达玛烷-24-烯-3β,20-二醇-3-乙酸酯（Ⅱ）、18-去甲基-5α,20ε-去氧胆酸,3β-20-二羟基-4,4,8,14-四甲基-γ-内酯,乙酸酯（Ⅲ）、20S,24S-环氧达玛烷-25-醇-3α-羟基乙酸酯（Ⅳ）、白桦酯酸（Ⅴ）、原儿茶酸（Ⅵ）、八氢-1H,5H-二吡咯[1,2-a：1′,2′-d]吡嗪（Ⅶ）。结论：化合物ⅠⅦ均为首次从连翘籽中分离得到,其中化合物Ⅶ为一种新的天然化合物。

Objective：To study the chemical constituents in the seed of Forsythia suspense.Methods： The compounds were isolated by column chromatography on silica gel and Sephadex LH-20;Their structures were identified by spectral data and other methods.Results： Seven compounds were isolated and identified as β-Amyrin acetate（Ⅰ）;20（S）-Dammar-24-ene-3β,20-diol-3-acetate（Ⅱ）;18-nor-5α,20ε-cholanic acid,3β-20-dihydroxy-4,4,8,14-tetramethyl-γ-lactone,acetate（Ⅲ）;20S,24S-epoxydammarane-25-ol-3α-yl acetate（Ⅳ）;betulinic acid（Ⅴ）;protocatechuic acid（Ⅵ）;octahydro-1H,5H-dipyrrolo[1,2-a：1′,2′-d]pyrazine（Ⅶ）.Conclusion： CompoundⅠ～Ⅶ are isolated from the seed of Forsythia suspense for the first time,and compound Ⅶ is a new natural compound.