人参皂苷Rg_3对人肺鳞癌细胞VEGF及KDR表达的影响

The Effects of Ginsenosides Rg3 on the Expressions of VEGF and KDR in Human Lung Squamous Cancer Cells

目的:探讨人参皂苷Rg3在抑制肿瘤新生血管生成过程中的作用,及其对人肺鳞癌SK-MES-1分泌的血管内皮细胞生长因子(VEGF)及其受体KDR的影响。方法:体外培养人肺鳞癌SK-MES-1细胞,经不同浓度的Rg3处理后,采用免疫细胞化学法与RT-PCR法检测Rg3对SK-MES-1细胞株VEGF mRNA、KDR mRNA及其蛋白表达的影响。结果:免疫细胞化学结果显示人参皂苷Rg3各浓度组SK-MES-1细胞VEGF及KDR蛋白阳性率与对照组比较差异显著。RT-PCR结果显示随着Rg3浓度的增加,VEGF、KDR扩增条带逐渐减弱,各浓度组与对照组比较差异显著。结论:人参皂苷Rg3能够抑制人肺鳞癌SK-MES-1细胞VEGF mRNA与KDR mRNA及其蛋白水平的表达,这可能是Rg3抑制肿瘤新生血管形成的机制之一。

Objective：To investigate the inhibitive effects of Ginsenosides Rg3（GS-Rg3）in the process of tumor angiogenesis and the effects on the expressions of VEGF and its receptor KDR in human lung squamous cancer SK-MES-1 cell line.Methods：Human lung cancer SK-MES-1 cells were cultured in vitro and immunocytochemistry and RT-PCR methods were used to detect the effects of different concentrations of Rg3 on the expressions of VEGF and KDR on SK-MES-1 cells.Results：The immunocytochemistry results showed that the positive rates of VEGF protein in different group of SK-MES-1 cells were 81.33±9.04,61.80±7.98,43.80±5.25,29.77±8.04,respectively.The positive rates of KDR protein in different group of SK-MES-1 cells were 65.51±7.45,51.73±9.21,34.87±6.15,22.04±5.11,respectively.There were significant differences between each group.RT-PCR results suggested that with the increase of the concentration of Rg3,VEGF and KDR amplified bands gradually weakened.There were significant differences between each group.Conclusion：GS-Rg3 can down-regulate the expressions of KDR and VEGF protein and their mRNA in human lung squamous cancer SK-MES-1 cells.It may be one of the mechanisms in the process of inhibiting tumor angiogenesis.