干姜、陈皮提取物对大鼠心肌缺血的影响

Effects of Rhizoma Zingiberis and Pericarpium Citri Reticulatae Extracts on Myocardial Ischemia in Rats

目的:观察干姜、陈皮提取物对结扎左冠状动脉前降支致急性心肌缺血模型大鼠的影响并初步探讨其作用机制。方法:建立大鼠心肌缺血模型,以复方丹参滴丸为阳性对照药,观察干姜、陈皮提取物配伍对大鼠心电图(ECG)、心肌梗死面积、血液流变学、乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的影响。结果:干姜、陈皮提取物组可改善ECG(P<0.01)、减轻心肌梗死程度(P<0.05)、减少LDH和CK的释出(P<0.01,P<0.05)、改善血液流变学指标(P<0.05),提高心肌组织SOD及GSH-Px的活力,降低MDA的含量(P<0.05)。结论:干姜、陈皮提取物对大鼠心肌缺血具有显著的保护作用,其机制可能与其抑制脂质过氧化反应有关。

Objective：To observe the effects of Rhizoma Zingiberis and Pericarpium Citri Reticulatae extracts on acute myocardial ischemia rats and explore the mechanism.Methods：The model of myocardial ischemia in rats was established by ligating the front descending anterior branch of the coronary artery.With Fufang Danshen Pill as positive control drug,the effects of Rhizoma Zingiberis and Pericarpium Citri Reticulatae extracts on the electrocardiogram（ECG）,the extension of myocardial infarction,the hemorheology indexes,lactic dehydrogenase（LDH）,creatine kinase（CK）,superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and malondialdehyde（MDA） in rats were evaluated.Results：Rhizoma Zingiberis and Pericarpium Citri Reticulatae extracts decreased the ST-segment of ECG（P〈0.01）,reduced the extension of myocardial infarction（P〈0.05）,decreased the contents of CK and LDH in serum（P〈0.01 or P〈0.05）,improved hemorheology（P〈0.05）,increased SOD and GSH-Px activity and decreased MDA content（P〈0.05）.Conclusion：Rhizoma Zingiberis and Pericarpium Citri Reticulatae extracts have protective effect on myocardial ischemia in rats,and its mechanism may be related to inhibiting lipid peroxidation.