柴胡疏肝散的提取工艺优化

Study on the Optimal Extraction Process of Chaihushugan Powder

目的:采用单因素考察和正交试验设计,对复方中药柴胡疏肝散提取工艺进行优化。方法:采用RP-HPLC法测定柴胡疏肝散中质量控制指标柴胡皂苷a、阿魏酸、橙皮苷、芍药苷的含量。以4个活性成分的含量测定结果与浸膏得率为考察指标,选用L9(34)正交表安排实验,对乙醇浓度、乙醇用量、回流时间、回流次数4个因素进行优化。结果:柴胡疏肝散最佳提取工艺为加入10倍量体积分数为80%的乙醇,回流提取2次,每次90 min。结论:本实验为柴胡疏肝散的制剂开发提供了实验依据。

Objective： To study the optimal extraction process of chaihushugan powder by orthogonal design.Methods： RP-HPLC method was developed for the determination of saikosaponin a,ferulic acid,hesperidin and paeoniflorin in chaihushugan powder.The contents of the components and the extraction yield were selected as assessment indices.Four factors were study by L9（34）,including the alcohol concentration,amount of alcohol,duration of extraction and times of extraction.Results： The optimal extracting condition was 80% alcohol consumed as 10 times of crude herb amount,and extrating two times for 90 min each time.Conclusion： This study supplies theoretical base for the development of chaihushugan powder formulation.