转座子Tn917插入位点侧翼序列的扩增

Amplification of flanking sequence of the transposon Tn917 insertion sites

为了克隆到生防菌株的抗病基因,以一株对灰葡萄孢菌表现很高拮抗活性的蜡样芽孢杆菌B-02菌株为材料,利用转座子标签技术得到拮抗性消失的突变体,进而利用TAIL-PCR技术扩增出Tn917插入位点两侧的未知序列,利用生物信息学分析扩增序列,为进一步研究该基因片段与菌株拮抗性之间的关系奠定了基础。

In order to clone the resistance genes of biocontrol strains, Bacillus cereus B-02 showed potentialy antagonistic activity against Botrytis cinerea was used as the material. The mutant was obtained by transposon tagging with antagonistic activity disappeared technology. Unknown flanking sequence of the transposon Tn917 insertion sites was amplified by TAIL-PCR methods. Bioinformation analyses were used to analyse amplified sequence. This research will help for further study on the relationship between this gene fragment and Bacillus cereus B-02 antagonistic.