甲壳素脱乙酰酶产生菌产酶条件的优化

Optimizing producing conditions of chitin deacetylase

采用斜面培养和液体发酵培养产甲壳素脱乙酰酶的真菌构巢曲霉,并且研究了产酶条件。结果表明,构巢曲霉的最适产酶条件为:发酵培养基初始pH值为6.5、发酵时间为96h、培养温度为31℃、碳源浓度为2%、氮源浓度为2%、金属离子浓度为0.01mol/L、接种量为6%。

Chitin deacetylase producing condition by Aspergillus nidulans was studied. The factors like the pH, fermenting time, temperature, the concentration of carbon and nitrogen sources, the addition of metal ions, the inoculation quantity of Aspergillus nidulans and production were selected as effecting of deacetylase activity. Result was obtained by orthogonal experiment design on the basis of single factor. The optimal condition for chitin deacetylase production was as follow： initial pH 6.5, carbon source 2%, nitrogen source 2%, the addition of metal ions 0.01mol/L, inoculun 6%, incubated at 31℃ for 96 h.