急性冠脉综合征患者淋巴毒素-α Ala252Gly多态性研究

Polymorphism of lymphotoxin-αAla252Gly in acute coronary syndrome

目的:探讨淋巴毒素-αAla252Gly多态性以及血清中淋巴毒素-α(LTA)水平在急性冠脉综合征(ACS)发病中的作用。方法:PCR-RFLP方法进行DNA多态性的检测,以分析LTA基因型以及基因频率分布。ELISA测定血清LTA水平。结果:ACS组中A/A基因型17例(16.04%),A/G基因型80例(75.47%),G/G基因型9例(8.49%);健康对照组A/A基因型38例(47.5%),A/G基因型34例(42.5%),G/G基因型8例(10.00%);ACS组LTA水平高于对照组(P<0.01)。结论:ACS组的LTA基因型与正常对照组的差异有显著性(χ2=23.46,P<0.01);LTA基因型与冠状动脉病变严重程度之间差异无显著性(χ2=3.64,P>0.05);ACS组的LTA血清浓度与正常对照组的差异有显著性(t=11.55,P<0.01)。

Objective： To study the effects of lymphotoxin-αAla252Gly polymorphism and soluble lymphotoxin-α＇ s level in blood serum in acute coronary syndrome （ACS） patients. Methods： The distribution of allele frequency and genotype frequency of LTA of all the samples were detected by using PCR- RFLP. The levels of LTA in serum were tested by using ELISA. Results： The quantity of subjects of A/A genotype, A/G genotype and G/G genotype of LTA in ACS group were 17 （16.04%）,80 （75.47%） and 9 （8.49%） respectively versus 38（47.5%）,34（42.5%） and 8（10.00%） in control group. The level of LTA in ACS group was significantly higher than that in control group （P〈0.01）. Conclusion：Difference of the genotype of LTA between the ACS group and the control group is significant statistically （Х^2=23.46,P〈0.01）;there is no significantly differences between the genotype of LTA and the serious of coronary artery（Х^2=3.64, P〉0.05 ）; difference of LTA＇ s concentration in serum between ACS group and control group is significant statistically （t=11.55 ,P〈0.01 ）.