摘要
目的：研究卡介苗（ＢＣＧ）６５ＫＤ热休克蛋白的提纯方法。方法：ＢＣＧ经３７℃恒温振荡培养两周，４２℃热休克处理２ｈ，培养液经离心、抽滤、盐析沉淀、ＳＤＳ－ＰＡＧＥ分离、电泳洗脱等步骤纯化。结果：提纯产物经ＳＤＳ－ＰＡＧＥ分析，其电泳区带在６５ＫＤ位置，经抑制试验表明具有较高抗原活性。结论：流程方法简单、实用，有助于对ＩＤＤＭ的诊断及其深入研究。
objective:To study the isolated and purified method of BCG 65KD beat shock protein.Method:BCG Was cultured at a constant temperature of 37℃with stirring sbake for two weeks,and then heat shock treatment at 42℃ for 2 hours,65KD HSP Was purified from the medium by centifugation,filtralion,saltiag -out rectpitation with ammonrum sultate,isolation with SDS-PAGE, elrtion with electrophoresis,etc.results:The purified protein was found to be a singie hand with mw 65KD by SDS-PAGE,and proved to be higher sntigen activity by blooking test.Conchosion:The procedure is simple,practical and do not need specific instrument and regeants,which will be helpful for the diagnosis of IDDM and its further research.
