异色瓢虫不同地理种群的ISSR种群多样性分析(英文)

Genetic diversity of populations of Harmonia axyridis(Coleoptera:Coccinellidae) from USA and China revealed by ISSR-PCR

异色瓢虫Harmonia axyridis(Pallas)在亚洲地区是一种重要的捕食性天敌昆虫,但是当引入到欧美后,对当地的物种产生了威胁。本文用ISSR技术对两个中国本地种群和一个引入美国的种群进行了种群多样性以及种群分化的分析。8条ISSR引物共扩增出105条带,其中多态性条带比率为96.19%。Nei's多样性指数、Shannon信息指数、多态性条带比率,在中国种群与美国种群中均非常接近,种群分化不明显(G_(st)=0.042)。AMOVA分析显示种群多样性主要来源于种群内部,种群间的基因流非常强(N_m=5.537)。基于Nei's多样性构建的聚类结果显示ISSR是一种快速有效的研究异色瓢虫种群多样性关系的技术。

The muhicoloured Asian ladybeetle, Harmonia axyridis （Pallas）, is a well-known aphid predator in its native Asian range and appears a threat to native species after imported into America and Europe. In this paper eight different ISSR primers have been used for analyzing population differentiation and genetic variations among one introduced population in America and two native Chinese populations. The resulting 105 loci showed a total polymorphism of 96. 19%. Genetic diversity estimated by Nei＇s gene diversity （He） , Shannon＇s information index （Ⅰ） and the percentage of polymorphism loci are similar in native and introduced populations. There is no obvious differentiation estimated by Nei＇s index （ Gst = 0. 0432） among populations. Most of the genetic variation is observed within populations （AMOVA： 91.22% and mean polymorphism of 94% ） and very high rates of gene flow were detected （Nm =5. 537） among populations. Phylogenetic dendrogram constructed based on Nei＇s genetic diversities suggested that ISSR is a quick and effective technique to study the relationship of geographic populations of H. axyridis.