凋亡相关基因Bcl-2、Bax在NSCLC中的表达与细胞凋亡的关系

Expression of apoptosis assotiated gene BCL-2,BAX and the relationship between expression and apoptosis in non-small-cell lung cancer

目的明确Bcl-2、Bax在NSCLC的发生、发展机制方面的作用。方法随机选择48例NSCLC手术标本及15例癌旁正常组织,应用免疫组化SP法检测Bcl-2、Bax的表达、TUNEL法检测细胞凋亡。结果NSCLC组Bcl-2、Bax、AI的表达均高于正常组织(P<0.05);Bcl-2、Bax、AI在不同病理分级、TNM分期、淋巴结是否转移组中比较差异有统计学意义(P<0.05);NSCLC中Bcl-2与AI成负相关关系(r=-0.609,P<0.05),Bax积分与AI成正相关关系(r=0.821,P<0.05)。结论Bcl-2可能参与了肺癌初期的发生、发展,抑制了癌组织的细胞凋亡;Bax与其发展、转移过程、进展中肺癌凋亡水平的升高有关。

Objective To identify the effect of Bcl-2/Bax on the mechanism of NSCLC and to detect two kinds of protein and apoptosis in NSCLC in the experiment. Methods 48 cases of NSCLC after operation in the near future and 15 cases of normal tissue were chosen randomly in this experiment. Bcl-2 and Bax were detected by immunohistochemistry SP technique, and the apoptosis was detected in TUNEL. Results Bcl-2, Bax, AI were all expressed higher in NSCLC than those in normal lung tissues（ P 〈 0. 05 ）. The positive points of Bcl-2, Bax, AI in differentiation grade, TNM stage, lymphatic metastasis had statistical significance （ P 〈 0. 05 ）. The relationship between Bcl-2 positive points and apoptosis indexes was negatively correlated （r = -0. 609, P 〈 0. 05 ）, but it was positively correlated between Bax positive points and apoptosis indexs （ r = 0. 821, P 〈 0.05 ）. Conclusion In this experiment, Bcl-2 has participated in the pristine genesis and development and has inhibited the apoptosis of NSCLC. Bax is concerned with the development, metastasis and the increase of apoptosis level in the progression of NSCLC.